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대한생식의학회지 제28권 제4호 2010년
Progesterone 및 Acetyl-L-Carnitine이 정자의 동결-융해에 미치는 영향
인제대학교 의과대학 일산 백병원 산부인과교실,김포 중앙 라산부인과
정병준, 정병준,김윤진,최형민,전명권,이응수,나오순
The Effects on Sperm Parameters and Membrane after Treatment with Progesterone and/or Acetyl-L-Carnitine; Cryopreservation-Thawing
Jung bj
Department of Obstetrics and Gynecology, College of Medicine, Inje University
Objective: To assess the effects of progesterone and acetyl-L-carnitine used after treated with Isolate® gradient before semen cryopreservation-thawing on sperm parameters and membrane integrity. Material and Methods: From April 2001 to July 2001, ten normal male partner of couples who were visited in vitro fertilization (IVF) clinics. the semens were treated with Isolate® gradient before cryopreservation, spermatozoa was incubated with progesterone (1, 5 and 10 μM), acetyl-L-carnitine (2.5, 5 and 10 μM), or both (progesterone, 1 μM; and acetyl-L-carnitine, 5 μM) for 30 min. Results: There were no differences in sperm parameters and vital stain among isolate only treated group, progesterone (1, 5 and 10 μM), acetyl-L-carnitine (2.5, 5 and 10 μM) and both (progesterone, 1 μM; and acetyl-L-carnitine, 5 μM). But, in high concentration of acetyl-L-carnitine (10 μM) treated group, sperm parameters and vital stain were decreased. The statistical method was used ANOVA (Kruskal-Wallis test) and p value was <0.01. Conclusions: Neither progesterone nor acetyl-L-carnitine show to be protective effect on the cryodamage assessed by sperm parameters and vital stain (eosin-Y stain) in normal sperm. High concentration of acetyl-L-carnitine (10 μM), however, was harmful effect on cryoprevention.
키워드 : Progesterone, Acetyl-L-carnitine, Sperm parameters, Cyropreservation
교신저자 : byeongjj@orgio.net
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