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대한생식의학회지 제31권 제4호 2010년
착상 전 생쥐배아에서 Id 유전자의 발현
울산대학교 서울아산병원 산부인과1,한양대학교 자연과학대학 생명과학과2,청주성모병원3
홍석호1, 나희영1,이영진1,2,이지원1,손영수3,채희동1
Expression of Ids in Preimplantation Mouse Embryos
1Dept of OBGY, College of Medicine, Ulsan University, Asan Medical Center, Seoul, Korea 2Dept of Life Science, College of Natural Sciences, Hanyang University, Seoul, Korea 3Cheong-Ju St. Marys' Hospital, Cheong-Ju, Korea
Objective: The Id family of helix-loop-helix proteins are thought to affect the balance between cell growth and differentiation by negatively regulating the function of basic-helix-loop-helix (bHLH) transcriptional factors. The aim of this study was to investigate the expression pattern of Ids (Id-1,-2,-3, and -4) in preimplantation mouse embryos at mRNA and protein levels. Methods: Oocytes and preimplantation embryos were collected from reproductive organs of female ICR mice following superovulation. RT-PCR was performed to investigate the mRNA expression patterns of Id genes and their protein were localized by immunofluorescence analysis. Results: Id-1 and Id-3 mRNAs were strongly expressed at the germinal vesicle (GV) oocyte and the blastocyst stages. Id-2 mRNA was expressed throughout preimplantation embryo development, but Id-4 was not expressed. Immunofluorescence showed that Id-1 and Id-2 were predominantly localized in cytoplasmic region, but the immunofluorescence signal of Id-3 was weak throughout preimplantation embryo development. Conclusion: These data show for the first time that Ids are expressed in preimplantation mouse embryos and suggest that Ids may play an important role in early preimplantation embryo development and uterine physiological changes.
키워드 : Id, Immunofluorescence, Mouse, Preimplantation embryo
교신저자 : shhong@amc.seoul.kr
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