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대한생식의학회지 제32권 제4호 2010년
체외수정시술 시 획득한 미성숙난자의 환자 연령에 따른 체외성숙률 및 수정률 비교
분당서울대학교병원 산부인과1, 서울대학교 의과대학 산부인과학교실2, 서울대학교 의학연구원 인구의학연구소3
한상훈1,, 이정렬2,김현준1,문정희1,지병철1,구승엽2,서창석1,2,3,김석현2,3,최영민2,3,김정구2,문신용2,3
Effects of Age on in vitro Maturation and Fertilization of Immature Oocytes from Stimulated Cycles in Human IVF-ET Program
Sang Hoon Han1,Jung Ryeol Lee2,Hyun Jun Kim1,Jung Hee Moon1,Byung Chul Jee1,Seung-Yup Ku2,Chang Suk Suh1,2,3,Seok Hyun Kim2,3,Young Min Choi2,3,Jung-Gu Kim2,Shin Yong Moon2,3
1Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Korea, 2Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea, 3Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul, Korea
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Objective: To investigate the effects of female age on in vitro maturation and fertilization of immature oocytes from controlled ovarian hyperstimulation (COH) in human IVF-ET program. Method: A total of 96 immature oocytes (GV & metaphase I) obtained from 40 cycles of IVF-ET (29 patients). The mean age of female patients was 31.8±3.1 years. Ovulation was triggered by urinary or recombinant hCG. Immature oocytes were cultured with YS medium containing 30% of patients' human follicular fluids, LH (1 IU/mL), FSH (1 IU/mL) and EGF (10 ng/mL), and then matured oocytes were fertilized by ICSI. In vitro maturation and fertilization of immature oocytes were analyzed according to age of female (< 34 or ≥ 34 years). Results: The maturation rate was similar between two groups (68% vs 64%). The fertilization rate of in?vitro-matured oocytes was higher in patients < 34 years old, but there was no statistical significance (64% vs 50%, p=0.347). The fertilization rate of in-vitro-matured oocytes was significantly lower compared with those of in-vivo-matured oocytes in both age groups (64% vs 79%, p=0.035, 50% vs 86%, p=0.007). Conclusion: In older female group, fertilization rate of in-vitro-matured oocytes seems to be decreased. Further investigations should be warranted to increase fertilization potential of in-vitro-matured oocytes.
키워드 : Immature oocyte, Female age, In vitro maturation, Controlled ovarian hyperstimulation, Fertilization
교신저자 : blasto@snubh.org
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