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대한생식의학회지 제27권 제3호 2010년
제 2일째 생쥐 배아의 초자화동결과 초급속동결
대학 산부인과1, 성신여자대학교 자연과학대학 생물학과2
양정숙1, 손철1, 배인하2,
Vitrification and Ultrarapid Freeze of Day 2 Mouse Embryos
Jung-Sook Yang1, Cherl Sohn1, In-Ha Bae2
1Taehak Infertility Clinic, 2Department of Biology, Collage of natural Sciences, Sungshin Women's University
Objective: This study was performed to compare the survival rate and the development of day 2 mouse embryos which had freezing procedures done. Methods: We used three different vitrification solutions (EFS, VS14, DPS) and a ultrarapid freezing solution (UFS) for cryopreservation of day 2 mouse embryo. Results: We tested toxicity by exposing embryos to vitirification solution and a ultrarapid freezing solution. The survival rates are 100%, 97.8%, 95.6% and 100% (EFS, VS14, DPS and UFS). After cultured for 96 hours, hatching rates of each group are 93.5% (no freezing), 95.6% (EFS), 86.4% (VS14), 93,0% (DPS), and 93.0% (UFS). There is no significant differences among groups. The survival rates after thawing cryopreserved embryos are 80.2%, 91.7%, 69.5%, 0% and 91.8% (slow freezing, EFS, VS14, DPS and UFS). Also cultured for 96 hours, the hatching rates are 93.5% (no freezing), 84.1% (slow freezing), 93.9% (EFS), 48.5% (VS14) and 70.1% (UFS). Conclusion: The survival rates of vitrification in EFS solution and ultrarapid freezing are higher than slow freezing (p<0.05). The hatching rate of vitrification in EFS solution cultured for 96 hours is highest, so vitrification of day 2 mouse embryos in EFS solution considered as more effective for cryopreservation.
키워드 : Day 2 mouse embryo, Vitrification, Ultrarapid freezing
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