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대한생식의학회지 제12권 제2호 2010년
체외에서 성숙시킨 토끼난자의 발생능력에 관한 연구
성신여자대학교 자연과학대학 생물학과;
배인하;,
The Attempts to Prove Normality of the Rabbit Follicular Oocyte Cultured In-Vitro
Bae, In-Ha;
Department of Biology, Sung Shin Women's University, College of Natural Sciences;
1) Rabbit follicular oocytes from preovulatory follicles were cultured for 12 hr in vitro and fertilized in vivo by transferring the oocytes to the first foster-mother. 2) Two youngs were bron from transferred embryos from the first foster-mother to the second foster-mother. This demonstrates that in vitro cultured follicular oocytes are normal and they can develop into normal young born when transferred to the foster-mother. 3) A simple chemically defined culture medium, salt sol. with glutamine (2mM), which was developed by Bae and Foote(1975) proves fully good enough for rabbit follicular oocyte culture. We call this B-F medium. 4) Twelve hours culture in vitro of the rabbit follicular oocyte may be a proper culture time for further development.
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