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대한생식의학회지 제13권 제2호 2010년
생쥐 Age에 따른 난자-난구 복합체의 분산과 성숙에 관한 연구
전남대학교 생물학과;전남대학교 생물학과;
이원교;권혁방;,
Cumulus Expansion and Oocyte Maturation of Oocyte-Cumulus Complexes Isolated from Different Ages of Mice In vitro
Lee, Won-Kyo;Kwon, Hyuk-Bang;
Department of Biology, Chonnam National University;Department of Biology, Chonnam National University;
In order to know when the cumulus cells of mouse follicles get ability to expand in vitro, the oocyte cumulus complexes obtained from different growing ages of mice were cultured in the medium containing HCG and their rate of expansion were observed and at the same time their maturation rate was examined. The growth of follicles was also checked by histological method. It was impossible to isolate the oocyte-cumulus complexes from 13 or 15 days old mouse ovaries. The oocyte-cumulus complexes collected from 17 days old mouse were partially induced to expanded by HCG, and from 19 days, most of the complexes were induced to full expansion. The rate of cumulus cell expansion by HCG and the oocyte maturation increased steadly during the growing ages to adult. Thus, the time for follicles to get competence for expansion and maturation seems to be closely related. Antral follicles were appeared from 17 days old mice and Graafian follicles were seen from 21 days old mice. The competence for cumulus expansion increased during follicle growth up to 21 days old mice.
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