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대한생식의학회지 제20권 제1호 2010년
백서에서 Serine Protease 억제제가 난포성숙에 미치는 영향에 대한 연구
서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;
윤병구;이진용;,
Effect of Serine Protease Inhibitor on Follicular Development in the Rat Ovary
Yoon, Byung-Koo;Lee, Jin-Yong;
Department of Obstertrics and Gynecology, College of Medicine, Seoul National University;Department of Obstertrics and Gynecology, College of Medicine, Seoul National University;
Plasminogen activator (PA)-plasmin system in follicular fluid is involved in the process leading to follicular rupture at ovulation. It is well known that PA is closely associated with cellular differentiation and tissue remodeling on evidences from the study of normal and malignant tissues. This study was designed to ascertain a potential role of PA in the ovarian folliculogenesis. Immature Sprague-Dawley rats were injected with pregnant mare serum gonadotropin, followed by injection of serine protease inhibitor (SPI; mixture of 1 mol/L benzamidine and 1 mol/L amino-caproic acid) into the unilateral ovarian bursa. In the control study, mechanical effect of bursal injection and contralateral ovarian effect SPI were ruled out. Total antral follicular areas relative to total ovarian cross-sectional areas was siginificantly lower in SPI-injected ovary than in saline-injected ovary. SPI injection decreased the relative antral follicular area by 33 % respectively. Electron microscopic finding of granulosa cell in the atretic follicle showed the presence of pyknotic nucleus, blurring of neucleolemma, degeneration of mitochondria and dilation of endoplasmic reticulum. After induction of ovulation with hCG, the number of oocytes released was significantly decreased in SPI-injected oviduct than in saline-injected oviduct. From above results, author discussed that PA may play a role not only in ovulation but also in some processes of folliculogenesis.
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