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대한생식의학회지 제20권 제1호 2010년
생쥐 배아 동결시 전핵의 발생시기가 생존률과 발생률에 미치는 영향
P.L. 산부인과 체외수정 연구실;P.L. 산부인과 체외수정 연구실;P.L. 산부인과 체외수정 연구실;P.L. 산부인과 체외수정 연구실;P.L. 산부인과 체외수정 연구실;P.L. 산부인과 체외수정 연구실;
양현원;강희규;최규완;차영범;이승재;박종민;,
Effects of the Age of Pronucleate Ova on Survival and Development in Cryopreservation of Mouse Embryos
Yang, Hyun-Won;Kang, Hee-Kyoo;Choi, Kyoo-Wan;Cha, Young-Beom;Lee, Seung-Jae;Park, Jong-Min;
IVF Research Laboratory, Park & Lee Infertility Clinic;IVF Research Laboratory, Park & Lee Infertility Clinic;IVF Research Laboratory, Park & Lee Infertility Clinic;IVF Research Laboratory, Park & Lee Infertility Clinic;IVF Research Laboratory, Park & Lee Infertility Clinic;IVF Research Laboratory, Park & Lee Infertility Clinic;
The effects of freezing and 1,2-propanediol on early and late pronucleate stage mouse ova were investigated in terms of survival after thawing and development in vitro. The samples were divided into two groups according to different age in pronucleate ova: ova in(1) early pronuclear stage with two distant pronuclei at 18h after hCG injection, and (2) late pronuclear stage with adjacent pronuclei at 30h. Zygotes in the late pronuclear stage have been proven to be more resistant to 1,2-propanediol, showing a significantly higher developmental rate than zygotes in early stage (80.3 versus 66.3%, <0.05), but survival rate was similar in the two groups (91.0 versus 93.5%). After freezing and thawing, survival and developmental rates were decreased in both groups when compared to the control group (54.3 versus 92.3%, 47.7 versus 73.3%. respectively). And developmental rate in the late pronuclear stage zygotes showed significantly higher than in early (55.4 versus 40.0%) after thawing. In conclusion, early pronucleate mouse ova have a lower developmental capacity in vitro and a lower survival rate after freezing and thawing than late ova. These findings suggest that the timing of freezing could be important for survival and further development in vitro in cryopreservation of human pronucleate ova.
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