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대한생식의학회지 제20권 제1호 2010년
Collagen Gel을 이용한 사람의 고환 조직배양에 관한연구
경희대학교 의과대학 비뇨기과학교실;경희대학교 의과대학 비뇨기과학교실;경희대학교 의과대학 비뇨기과학교실;경희대학교 의과대학 비뇨기과학교실;경희대학교 의과대학 비뇨기과학교실;경희대학교 의과대학 비뇨기과학교실;경희대학교 의과대학 비뇨기과학교실;
이충현;이상철;이선주;손준웅;장성구;김진일;채수응;,
A Study on Growth of Human Testicular Tissue in 3-Dimensional Collagen Gel Tissue Culture
Lee, Choong-Hyun;Lee, Sang-Cheol;Lee, Sun-Joo;Sohn, Joon-Woong;Chang, Sung-Goo;Kim, Jin-Il;Chai, Soo-Eung;
Department of Urology, School of Medicine, Kyung Hee University;Department of Urology, School of Medicine, Kyung Hee University;Department of Urology, School of Medicine, Kyung Hee University;Department of Urology, School of Medicine, Kyung Hee University;Department of Urology, School of Medicine, Kyung Hee University;Department of Urology, School of Medicine, Kyung Hee University;Department of Urology, School of Medicine, Kyung Hee University;
A recently developed collagen gel culture technique has been applied to study on growth in tissue of human testicular tissue. Minimum Eagle's medium supplemented with amino acid, 10% Fetal Bovine Serum and 0.1mM non-essential amino acid are emploid. Tissue fragments on collagan gel are fixed at time intervals for the histologic findings of testis. The mature spermatids are maintained for 2 weeks and can be observed until four weeks. But the rate of glucose consumption is increased contrary to histologic findings.
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