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대한생식의학회지 제21권 제1호 2010년
생쥐 난자 배양시의 자발적 투명대 경화 현상과 PMA에 의한 투명대 경화 현상 비교
성신여자대학교 자연과학대학 생물학과;성신여자대학교 자연과학대학 생물학과;서울여자대학교 자연과학대학 생물학과;
강혜나;배인하;김해권;,
The Comparison of the Spontaneous Zona Pellucida Hardening and PMA-induced Zona Pellucida Hardening during in Vitro Culture of the Mouse Oocytes
Kang, Hye-Na;Bae, In-Ha;Kim, Hae-Kwon;
Department of Biology, College of Natural Sciences, Sungshin Women's University;Department of Biology, College of Natural Sciences, Sungshin Women's University;Department of Biology, College of Natural Sciences, Seoul Woman's University;
One consequence of fertilization in mammals is an increased resistance of the zona pellucida (ZP) to proteases and various chemical reagents. This phenomenon has been called 'zona pellucida hardening' (ZPH), and it is generally accepted that it is caused by the secretory products of cortical granules released by the egg at fertilization. ZP of mouse oocytes maturing in vitro in a chemically defined medium becomes progressively more resistant to solubilization by chymotrypsin ("Spontaneous" ZP hardening). In the present study, it was aimed to find the specificity of spontaneous ZPH in relation to its possible relevance to the cortical reaction and the physiological block to polyspermy. When a maturation inhibitors, cAMP analog(dbcAMP) and phosphodiesterase inhibitor (IBMX) was added to culture medium, it prevent spontaneous ZPH of mouse oocyte during in vitro culture. Thus spontaneous ZPH requires GVBD, since it is prevented by those agents, which inhibit GVBD in vitro. However, culture for 3 hours in the presence of PMA(lOng/ml), a protein kinase C activator, resulted in ZPH without GVBD, thus suggesting that ZPH may be regulated independently apart from the event of GVBD. Pretreatment of mouse oocyte with FBS result in partially inhibitory effect on subsequent spontaneous ZPH. Induction of GVBD in vivo had a inhibitory effect on the spontaneous ZPH, but subsequent spontaneous ZPH. Induction of GVBD in vivo had a inhinbitory effect on the spontaneous ZPII, but had no inhibitory effect on PMA-induced ZPH. Treatment with a microfilament formation blocker(cytochalasin-B) at 1
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