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대한생식의학회지 제21권 제1호 2010년
성선자극호르몬이 흰쥐 난소의 GnRH와 GnRH mRNA의 발현에 미치는 영향
경상대학교 의과대학 산부인과학교실;경상대학교 의과대학 산부인과학교실;경상대학교 의과대학 산부인과학교실;경상대학교 의과대학 산부인과학교실;경상대학교 의과대학 산부인과학교실;경상대학교 의과대학 산부인과학교실;경상대학교 의과대학 해부학교실;경상대학교 의과대학 해부학교실;
백원영;정파진;박신근;김완영;이종학;김종화;김명옥;최완성;,
Effect of Gonadotropin on the Expression of GnRH and GnRH mRNA in Rat Ovary
Paik, Won-Young;Chung, Pa-Jin;Park, Shin-Keun;Kim, Wan-Young;Lee, Jong-Hak;Kim, Jong-Hwa;Kim, Myeong-Ok;Choi, Wan-Sung;
Department of Obstetrics, College of Medicine, Gyeongsang National University;Department of Obstetrics, College of Medicine, Gyeongsang National University;Department of Obstetrics, College of Medicine, Gyeongsang National University;Department of Obstetrics, College of Medicine, Gyeongsang National University;Department of Obstetrics, College of Medicine, Gyeongsang National University;Department of Obstetrics, College of Medicine, Gyeongsang National University;Department of Gynecology, Anatomy, Gyeongsang National University;Department of Gynecology, Anatomy, Gyeongsang National University;
Expression of gonadotropin releasing hormone(GnRH) has been described in the rat ovary. It remains, however, unkown whether GnRH is synthesized as a prohormone. Therefore, this study was performed to verify the expression of pro-GnRH by in situ hybridization and further to investigate the effect of gonadotropin on GnRH or GnRH mRNA in rat ovary by immunohistochemical and in situ hybridization techniques. Adult female Sprague-Dawely rats were used and the estrous cycle was synchronized by intraperitoneal injection of pregnant mare's serum gonadotropin(PMSG). Ovaries were fixed with 4% paraformaldehyde and embedded with G.C.T. compound and cut by cryostat. For immunohistochemistry, avidin-biotin peroxidase complex(ABS) method was employed and for in situ hybridization,
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