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대한생식의학회지 제21권 제2호 2010년
혈액세포를 이용한 염색체 분리 분석에 관한 방법적 고찰
진주산업대학교 낙농자원학과;한국생명과학연구소;
손시환;정구민;,
A Study on the Methodology of Chromosome Preparation from Blood Culture
Sohn, S.H.;Chung, K.M.;
Department of Dairy Science & Technology, Chinju National University;Hankook Institute of Life Science;
This study was carried out to develop the methodology of chromosome preparation from blood cultures in mammals which included human, mouse, cattle and pig. For karyotyping, 0.5-5.0ml of peripheral blood were collected and cultured. The satisfactory results were obtained from macroculture and microculture in all species. In culture, the patterns of cell growth were no difference among media except serum concentration and mitogen supplement. The presence of mitogen and fetal bovine serum in medium significantly affected the mitotic index. The optimal culture condition was 37
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