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대한생식의학회지 제27권 제1호 2010년
공배양의 작용기전에 관한 연구
부산대학교 의과대학 산부인과학교실, 부산대학교 자연과학대학 분자생물학과;문화병원 불임클리닉, 부산대학교 자연과학대학 분자생물학과;부산대학교 자연과학대학 분자생물학과;문화병원 불임클리닉;부산대학교 의과대학 산부인과학교실;부산대학교 자연과학대학 분자생물학과;
김미경;주보선;김미선;문화숙;이규섭;김한도;,
Mechanism for the Action of Co-culture
Kim, Mi-Kyoung;Joo, Bo-Sun;Kim, Mi-Sun;Moon, Hwa-Sook;Lee, Kyu-Sup;Kim, Han-Do;
Department of Obstetrics and Gynecology, Pusan National University School of Medicine, Department of Molecular Biology, Pusan National University School of Natural Science;The center of Reproductive Medicine and Infertility, Moon Hwa Hospital, Department of Molecular Biology, Pusan National University School of Natural Science;Department of Molecular Biology, Pusan National University School of Natural Science;The center of Reproductive Medicine and Infertility, Moon Hwa Hospital;Department of Obstetrics and Gynecology, Pusan National University School of Medicine;Department of Molecular Biology, Pusan National University School of Natural Science;
Objective: A number of studies to improve in vitro culture conditions have been tried over past ten years by using co-culture system with helper somatic cells. However, the mechanism of coculture is poorly understood. This study was designed to understand the mechanism for the mode of actual action of co-culture using co-culture system of ICR strain's 1-cell embryos with human oviduct epithelial cells by examining the effect of conditioned medium and contactless coculture using a cell culture insert on the embryo development and by measuring the level of superoxide anion from conditioned medium after co-culture. Methods: ICR strain's zygote embryos were cultured in medium alone (control), coculture, conditioned medium, or contactless coculture system for 6 days. Conditioned media (CM) were prepared as following 5 groups. All CM were collected after culturing oviduct cells for 2 days. CM-1 was stored at
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