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대한생식의학회지 제27권 제3호 2010년
Extracellular Matrix 배지에서 생쥐 배아의 발생 및 아폽토시스
울산대학교 의과대학 산부인과;경기대학교 자연과학부 생물학과;경기대학교 자연과학부 생물학과;바이오메드 연구소;경기대학교 자연과학부 생물학과;
강병문;손인표;정병목;최규완;계명찬;,
Preimplantation Development and Apoptosis of Mouse Embryos in the Medium Containing Extracellular Matrix
Kang, Byung-Moon;Sohn, In-Pyo;Chung, Byung-Mok;Choi, Kyoo-Wan;Gye, Myung-Chan;
Department of Obsterics and Gynecology, College of Medicine, Ulsan University;Department of Biology, Natural Sciences, Kyonggi University;Department of Biology, Natural Sciences, Kyonggi University;Biomed Institute;Department of Biology, Natural Sciences, Kyonggi University;
Objective: To verify the effect of Matrigel, a ECM complex from Engelbreth-Holm-Swarm (EHS) mouse sarcoma on the preimplantation development and apoptosis of mouse fertilized eggs. Method: Late pronucleus stage eggs were cultured through the blastocyst stage in the presence of Matrigel (0.5%, v/v). Characteristics of apoptosis and cell number assesed by Hoecst staining and TUNEL labeling at the blastocyst stage, respectively. Results: Morphological development, number of cells per embryo was significantly increased but rate and number of TUNEL positive nuclei of the embryo were decreased in the presence of Matrigel. Conclusion: This result suggested that at low concentration of Matrigel improves both viability and morphological development in the preimplantation mouse embryos.
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