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대한생식의학회지 제36권 제1호 2010년
정자 운동성 및 수명 보존을 위한 최적 배양에 관한 연구
중앙대학교 산업과학대학 동물자원과학과1, 생명환경연구원2
유영아1, EA Mohamed1, 오신애1, 방명걸1,2*,
Optimized Methods to Maintain Motility and Viability in Normozoospermic Males
Young-Ah You1, E.A. Mohamed1, Shin-Ae Oh1, Myung-Geol Pang1,2*
1Department of Animal Science & Technology and 2BET Research Institute, Chung-Ang University
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Objectives: To determinate the optimal culture condition to maintain lifespan in human sperm, we evaluated the effect of different temperature on sperm motility and viability up to 5 days in normal specimens. Methods: Ejaculated semen samples with normal semen parameters were gently washed in HEPES buffered Tyrod's-Albumin¬Lactate-Pyruvate (HTALP) media. Each 5 ml of HTALP + 0.3% albumin with 1×106 sperm/ml was incubated for 5 days in 37℃, 22℃, and 4℃. The sperm motility and kinematics were analyzed using computer assisted sperm analysis (CASA), membrane integrity was assessed by hypoosmotic swelling test (HOST), and capacitation status was evaluated by chlorotetracycline (CTC) fluorescence pattern. Each parameter was measured on day 1, 3, and 5, respectively. Results: The motility, viability and live/uncapacitated pattern were demonstrated significantly in temperature- and time-dependent difference (p<0.05). While the sperm cultured for 1 day in each temperature was not significantly different, the sperm cell kept in 22℃ after 3 days were preserved sperm motility, viability, membrane integrity, and F pattern better than in other culture temperatures. Conclusions: HTALP can be used a basic medium for culture and longevity preservation, and sperm cell kept at 22℃ is beneficial for assisted reproductive techniques.
키워드 : Optimal sperm culture condition, Longevity, Motility, Preservation
교신저자 : mgpang@cau.ac.kr
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