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대한생식의학회지 제25권 제3호 2010년
흰쥐 자궁에서 스테로이드호르몬에 의한 c-Fos, CREB, ATF 및 HSP70의 발현에 관한 연구
이화여자대학교 의과대학 의학과 및 의과학 연구소 분자생물학부, 제주대학교 의과대학 조직학교실
이영기, 김성례 ,
Effect of Steroid Hormones on the Expression of c-Fos, CREB, ATF, and HSP70 in Rat Uterus
Lee yk, Kim sr
Steroid hormone is known to causee the dynamic changes of mamalian uterus during reproductive cycle. However there is littl information about the effect of estrogen (E) and progesterone (P) on the expression of various transcription factors involved in gene expression. Thus the present study was designed to demonstrate E and/or P-induced expression of c-Fos, CREB, ATF and HSP70 in rat uterus. Rats, ovariectomized (OVX) for two weeks, were divided into 6 experimental groups, 1) OVX, 2) OVX+V, 3) OVX+E, 4) OVX+P, 5) OVX+E+V, 6) OVX+E+P, and western blotting assay for nuclear extract and immunohistochemical staining were carried out for each experimental group. Treatment of E (10μg) showed to increase the expression of c-Fos, CREB, ATF, and HSP70, and maximal expression was occured at 3-6 hr after E administration. P (1mg) also increased, but much less than E, the expression of c-Fos, ATF, and HSP70. However, P did not reveal any effect on the expression CREB. P treatment 4 hr after E injection decreased c-Fos, CREB, and ATF expression, but did not show any change in the E-induced HSP70 expression. In immunohistochemical study c-Fos-, CREB-, and ATF-immunoreactivities were confined to the cells of luminal epithelium of uterine endometrium. These results suggest that proliferation and differentiation of rat uterus during reproductive cycle may mediated via expression of transcription factors, such as c-Fos, CREB, ATF, and HSP70.
키워드 : Uterus, Ovariectomy, Steroid hormone, Transcription factor, Western Western blotting, Immunohistoch
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