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대한생식의학회지 제28권 제1호 2010년
체외배양 생쥐정소세포에서 합성에스트로젠이 P450동위효소의 발현에 미치는 영향
을지의과대학교 생리학교실1,의과학연구소2,서울보건대학교 임상병리과3
이호준1,김묘경2,고덕성2,강희규3,김동훈2, -
Effects of xenoestrogens on gene expression of cytochrome P450 genes in in vitro cultured mice spermatogenic cells
HJ Lee1,MK Kim2,DS Ko2,HK Kang3,DH Kim2
partment of Physiology 1 ,Eulji University DeSchool of Medicine,Medical Science
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Objective :To know the effects of xenoestrogen on spermatogenesis,we investigated the expression of cytochrome P450s enzymes (CYPscc,CYP17 α,CYP19) and 3 β-HSD genes involved in steroidogenesis. Methods :Mouse testicular cells were prepared from 15-day-old ICR mice which had only pre-meiotic germ cells by enzyme digestion using collagenase and trypsin. Testicular cells were cultured in DMEM supplemented with FSH (0.1 IU/ml)and 10%FBS or medium with estrogen (E2),bisphenol-A (BPA),octylphenol (OP;10-9,10-7,10-6,10 -5,10-4M,respectively)and aroclor 1254 (A1254)known as PCBs for 48 hours.The gene expression of cytochrome P450 enzymes were examined by semi-quantitive RT-PCR.The production of estrogen and testosterone was examined by RIA. Results :As a results,expression of CYPscc mRNA was not significantly decreased, but 3 β-HSD and CYP17 αmRNA were significantly dose-dependent decreased.And production of testosterone and estrogen were not different except BPA and OP group (10 -5 M). Conclusion :BPA,OP and A1254 might inhibit steroidogenesis by decreasing the CYPscc,3 β-HDS and CYP17 αmRNA expression in the mouse testis.These results suggest that BPA,OP and PCBs like as an endocrine disruptors inhibit the productions of steroidogenic enzymes and decrease the production of T and E by negative feedback mechanism.Therefore,these might disrupt steroidogenesis in Leydig cells of testis and would disturb testicular function and subsequently impair spermatogenesis.
키워드 : Mouse,Bisphenol-A,Octylphenol,PCB (Aroclor 1254),Cytochrome
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