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대한생식의학회지 제30권 제1호 2010년
인간자궁내막의 탈락막화 (Decudualization)에 있어서 TGF-β (Transforming Growth Factor-β)의 역할
아주대학교 대학원 분자과학기술학과, 아주대학교 의과대학 산부인과학교실, 아주대학교 자연대학 생명과학과
박동욱, 최동순,김미란,황경주,조미영,안성희,민철기,유희석
Transforming Growth Factor-β is a Possible Paracrine Mediator in the Human Endometrial Decidualization
Park DW
Department Molecular Science and Technology, Department of Obstetrics and Gynecology, School of Medicine, Department of Biological Science, Ajou University, Suwon, Korea
Objectives: To investigate the role of TGF (Transforming growth factor-β) involved in the paracrinic communication during decidualization between UEC (uterine epithelial cells) and USC (uterine stromal cells), we have employed a co-culture system composed of human endometrial epithelial and stromal cells in defined hormonal conditions. Design: In the co-culture, endometrial epithelial cells cultured in the matrigel-coated cell culture insert are seeded on top of the endometrial stromal cells cultured within a collagen gel. The co-culture was maintained for 48 hours under the following hormonal conditions: progesterone dominant condition (100 nM P4 and 1 nM E2) or estrogen-dominant condition (100 nM E2 and 1 nM P4). 10 ng/ ml HGF and/or 10 ng/ml TGF-β1 are added. Methods: RT-PCR is utilized to detect mRNAs quantitatively. Enzyme-linked immunosorbent assay (ELISA) and immunohistochemical staining are utilized to detect proteins in the tissue. Results: Prolactin mRNA is expressed in the co-cultured stromal cells under the progesterone dominant condition. TGF-β1 and its receptors are expressed in both the co-cultured epithelial and stromal cells irrespective of the steroid present, which is in contrast with no or negligible expression of TGF-β1 or its receptor in cells separately cultured. Both estrogen and progesterone significantly elevate the concentration of hepatocyte growth factor (HGF) in the conditioned medium of the co-culture with the value of 4,325 pg/ml in E2-dominant and 2,000 pg/ml in P4-dominant condition compare to 150 pg/ ml in no hormone. In separately cultured stromal cells, administration of HGF induces the expression of TGF receptor 1 in both hormonal conditions, but induction of TGF receptor 2 is only manifest in the P4-dominant condition. Administration of TGF-β and HGF directly induce the decidualization marker prolactin mRNA in separately cultured stromal cells. Conclusion: It is likely that steroid hormones induces prolactin mRNA indirectly by promoting the cell to cell communication between the stromal and the epithelial cells. TGF-β and HGF are two possible paracrine mediators in the human endometrial decidualization.
키워드 : Decidualization, TGF-β, HGF, Co-culture, Paracrine
교신저자 : kmr5300@ajou.ac.kr
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