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대한생식의학회지 제30권 제1호 2010년
착상전 생쥐 배아 발달에 대한 Apoptotic Gene의 발현
전남대학교 의과대학 산부인과학교실, 호르몬연구센터
이여일, 이진,김미영,전상영
Expression of Apoptotic Genes in Mouse Preimplantation Embryo Development
Lee YI
Department of Obstetrics and Gynecology, College of Medicine, 2Hormone Research Center, Chonnam National University, Gwangju, Korea
Objective: The aim of this study was to evaluate the influence of three different media on preimplatation embryo development and the expression of Bcl-2, Mcl-1, Bax, and Bok in mouse. Materials and Methods: Two-cell embryos were retrieved from ICR female mice (4 weeks old) at 48 hr after hCG injection and cultured in Ham's F-10, HTF, and G1.2 media. The developmental rate of 2-cell embryos was evaluated from 24 hr to 72 hr after culture. RT-PCR was performed for the detection of Bcl-2, Mcl-1, Bax, and Bok gene expression. Results: The rates of morula and blastocyst in HTF and G1.2 media (88%, 98.1%) were significantly higher than those in Ham's F-10 media (39.6%) at 48 hr. Likewise, the rates of hatching and hatched blastocyst in HTF and G1.2 media (21.9%, 52.9%) were higher than those in Ham's F-10 media (3.5%) at 72 hr. Bcl-2 and Bax mRNAs were highly detected in embryos cultured in Ham's F-10 when compared in embryos cultured in HTF and G1.2. In contrast, the expression of Mcl-1 and Bok was not significantly different. Conclusion: These results show that HTF and G1.2 culture media increase the rate of blastocyst formation and stimulate Bcl-2 and Bax gene expression in mouse preimplantation embryos.
키워드 : Blastocyst, Culture medium, Apoptotic gene, Preimplantation embryo
교신저자 : leeyi@chonnam.ac.kr
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