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대한생식의학회지 제30권 제4호 2010년
Mobile transposon-like element, clone MTi7: RNA interference를 이용한 역할 규명1
차병원 여성의학연구소,포천중문의과대학교 생명과학전문대학원,충북대학교 농과대학 축산학과,서울대학교 생명과학부
박창은, 신미라,전은현,조성원,이숙환,김경진,김남형,이경아
Mobile transposon-like element, clone MTi7: Finding its role(s) by RNA interference1
Park CE
Infertility Medical Center,CHA General Hospital.Graduate School of Life Science and Biotechnology, Pochon CHA Univ.Dep of Animal Science, Chungbuk National Univ,School of Biological Sciences SNU
Objectives: The present study was conducted to evaluate the mobile transposon-like element, clone MTi7 (MTi7) expression in the mouse ovary and to determine its role(s) in the mouse oocytes by RNA interference (RNAi). Methods: MTi7 mRNA expression was localized by in situ hybridization in day5 and adult ovaries. Double stranded RNA (dsRNA) was prepared for c-mos, a gene with known function as control, and the MTi7. Each dsRNA was microinjected into the germinal vesicle (GV) stage oocytes then oocyte maturation and intracellular changes were evaluated. Results: In situ hybridization analysis revealed that MTi7 mRNA localized to the oocyte cytoplasm from primordial to preovulatory follicles. After dsRNA injection, we found 43-54% GV arrest of microinjected GV oocytes with 68%-90% decrease in targeted c-mos or MTi7 mRNA. Conclusions: This is the first report of the oocyte-specific expression of the MTi7 mRNA. From results of RNAi for MTi7, we concluded that the MTi7 is involved in the germinal vesicle breakdown in GV oocytes, and MTi7 may be implicated with c-mos for its function. We report here that RNAi provides an outstanding approach to study the function of a gene with unknown functions.
키워드 : MTi7, Mouse, Oocyte Maturation, RNA Interference
교신저자 : leeka@nuri.net
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