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대한생식의학회지 제31권 제1호 2010년
Tyrosine Hydroxylase 유전자가 주입된 인간 배아줄기세포의체외 신경세포 분화
마리아 기초의학연구소/마리아바이오텍, 서울대학교 약리학교실, 마리아불임병원
신현아, 김은영,이금실,조황윤,김용식,이원돈,박세필,임진호
In vitro Neural Cell Differentiation of Genetically Modified Human Embryonic Stem Cells Expressing Tyrosine Hydroxylase
Hyun Ah Shin, Eun Young Kim, Keum Sil Lee, Hwang Yoon Cho, Yong Sik Kim, Won Don Lee, Sepill Park, Jin Ho Lim
Maria Infertility Hospital Medical Institute/Maria Biotech, Seoul National University, Dept. of Pharmacology, Maria Infertility Hospital
Objective: This study was to examine in vitro neural cell differentiation pattern of the genetically modified human embryonic stem cells expressing tyrosine hydroxylase (TH). MaterialsandMethods: Human embryonic stem (hES, MB03) cell was transfected with cDNAs cording for TH. Successful transfection was confirmed by western immunoblotting. Newly transfected cell line (TH#2/MB03) was induced to differentiate by two neurogenic factors retinoic acid (RA) and b-FGF. Exp. I) Upon differentiation using RA, embryoid bodies (EB, for 4 days) derived from TH#2/MB03 cells were exposed to RA (10－6 M)/AA (5×10－2 mM) for 4 days, and were allowed to differentiate in N2 medium for 7, 14 or 21 days. Exp. II) When b-FGF was used, neuronal precursor cells were expanded at the presence of b-FGF (10 ng/ml) for 6 days followed by a final differentiation in N2 medium for 7, 14 or 21 days. Neuron differentiation was examined by indirect immunocytochemistry using neuron markers (NF160 & NF200). Results: After 7 days in N2 medium, approximately 80% and 20% of the RA or b-FGF induced Th#2/MB03 cells were immunoreactive to anti‐NF160 and anti-NF200 antibodies, respectively. As differentiation continued, NF200 in RA treated cells significantly increased to 73.0% on 14 days compared to that in b-FGF treated cells (53.0%, p<0.05), while the proportion of cells expressing NF160 was similarly decreased between two groups. However, throughout the differentiation, expression of TH was maintained (~90%). HPLC analyses indicated the increased levels of L-DOPA in RA treated genetically modified hES cells with longer differentiation time. Conclusion: These results suggested that a genetically modified hES cells (TH#2/MB03) could be efficiently differentiated in vitro into mature neurons by RA induction method.
키워드 : Human embryonic stem cell, TH, Differentiation, RA, b-FGF
교신저자 : sppark@mariababy.com
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