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대한생식의학회지 제31권 제4호 2010년
난포자극호르몬이 인간의 자궁 기질세포의 유전자 발현 양상에 미치는 영향
성균관대학교 의과대학 삼성제일병원 생식생물학 및 불임연구실1,산부인과2,서울대학교 수의과대학 공중보건학교실3
최혜원1, 전진현1*,이형송1,홍인선3,강경선3,궁미경2
Alteration of Gene Expressions in Human Endometrial Stromal Cells by Exogeneous FSH Treatments
Hye Won Choi1, Jin Hyun Jun1*, Hyoung Song Lee1, In-Sun Hong3,Kyung-Sun Kang3, Mi Kyoung Koong2
1Laboratory of Reproductive Biology & Infertility,2Department of Ob/Gyn, Samsung Cheil Hospital, Sungkyunkwan University School of Medicine, Seou, Korea 3Department of Veterinary Public Health, College of Veterinary Medicine Seoul National University, Seoul, Korea
Objective: To evaluate the effects of recombinant FSH (rFSH) and urinary FSH (uFSH) on the gene expressions of human endometrial stromal cells in vitro. Methods: Endometrial tissue was obtained from a pre-menopausal women undergoing hysterectomy. Primary endometrial stromal cells were isolated and in vitro cultured with FBS-free DMEM/F-12 containing 0, 10, 100, and 1,000 mIU/ml of rFSH and uFSH for 48 hours, respectively. Total RNA was extracted from the cultured cells and subjected to real time RT-PCR for the quantitative analysis of progesterone receptor (PR), estrogen receptor α/β (ER-α/β), cyclooxygenase 2 (Cox-2), leukemia inhibitory factor (LIF), homeobox A10-1 and -2 (HoxA10-1/-2). Results: Both hormone treatments slightly increased (< 3 folds) the expressions of PR, ER-β and HoxA10-1/-2 gene. However, ER-α expression was increased up to five folds by treatments of both FSH for 48 hours. The LIF expression by the 10 mIU/ml of uFSH for 12 hours was significantly higher than that of rFSH (p<0.01). After 24 hours treatment of two kinds of hormones, the expression patterns of LIF were similar. The 100 and 1,000 mIU/ml of rFSH induced significantly higher amount of Cox-2 expression than those of uFSH, respectively (p<0.05). Conclusion: This study represents no adversely effect of exogeneous gonadotropins, rFSH and uFSH, on the expression of implantation related genes. We suggest that rFSH is applicable for the assisted reproductive technology without any concern on the endometrial receptivity.
키워드 : Recombinant FSH, Urinary FSH, Endometrial stromal cell, Implantation related genes, Endometrial receptivity *본 연구는 Organon 사의 연구비 지원 (GL 071)으로 수행되었습니다.
교신저자 : junjh55@hanmail.net
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