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대한생식의학회지 제32권 제2호 2010년
초자화동결된 생쥐 Preantral Follicle의 체외성장과 배란
경상대학교 의과대학 산부인과학교실1, 경상대학교 건강과학연구원2
박지권1,, 백원영1,2
In vitro Follicular Growth and Ovulation of Mouse Preantral Follicles Cryopreserved by Vitrification
Ji Kwon Park1, Won Young Paik1,2
1Department of Obstetrics and Gynecology, College of Medicine, Gyeongsang National University, JinJu, Korea, 2Gyeongsang Institute of Health Sciences, JinJu, Korea
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Objective: To define an appropriate vitrification condition of preantral follicle that yields high survival and to evaluate growth and ovulation rate of mouse follicles during in vitro culture after vitrification. Methods: Preantral follicles were isolated mechanically from mouse ovaries that were surgically recovered from mice aged 14 days. Retrieved preantral follicles were placed in EG (Ethylene Glycol) for 2, 5, 10 minutes and transferred to EFS-40 (40% EG, 18% Ficoll-70, 0.5 M sucrose) for 0.5, 1, 2 minutes. And then, preantral follicles were placed onto an EM grid and submerged immediately in liquid nitrogen. Thawing was carried out at room temperature. After defining the most appropriate vitrification condition that yields high survival, in vitro growth and ovulation rate of follicles were evaluated. Results: Appropriate vitrification condition that yield high survival rate (83.2¡¾2.1%) of preantral follicle was EG for 5 minutes and EFS-40 for 0.5 minutes. In vitro survival rate of the vitrified preantral follicles were 85.5¡¾0.5%, 67.9¡¾0.8% and 40.2¡¾0.5% on day 2, 6 and 10. And in vitro growth of the vitrified preantral follicles were 107.1¡¾16.1 µm, 117.1¡¾18.4 µm, 178.4¡¾45.6 µm and 325.4¡¾54.4 µm on day 0, 2, 6 and 10. Although in vitro survival rate and growth of vitrified preantral follicles were lower than that of non-vitrified preantral follicles, the patterns of survival and growth were similar in vitrified and non-vitrified preantral follicles. The ovulation rate of antral follicles that was grown from vitrified preantral follicles was 32.6¡¾1.2%. Conclusion: Vitrified preantral follicles could be grown to antral sizes, and mature oocytes that can be used for IVF-ET programs were produced successfully. These data suggest that cryopreservation of preantral follicle by vitrification can be used for the preservation of the fertility.
키워드 : Preantral follicle, Vitrification, Preservation of the fertility
교신저자 : wypaik@gshp.gsnu.ac.kr
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