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대한생식의학회지 제27권 제2호 2010년
사람과 흰쥐의 고환에서 Aquaporin 유전자의 발현
부산대학교 외과대학 비뇨기과학교실, 생리학교실
박남철, 박영수, 오금수, 정진섭,
Molecular Indentification and Distribution of Aquaporins in Human and Rat Testes
Nam Cheol Park, Young Soo Park, Gom Su Oh, Jin Sup Jung
Department of Urology and physiology, college of medicine, Pusan National University
Objective: Several water channels (aquaporins; AQP) that belon to the Mip (major intrinsic protein) family have identified. In the selected tissues including red blood cells or renal tubules, water movements are abundant and/or physiologically important. Unexpectedly, a high water AQP8 are present in testes, so that the high water permeability of human sperm suggested to be mediated by AQPs. Method: To identify the identity of aquaporins expressed in testes, RT-PCR performed using degenerative primers, which were designed to correspond to highly conserved sequences surrounding the Asn-Pro-Ala (NPA) motifs in the aquaporins. New expressed AQP series were reconfirmed by immunohistochemical study using rabbit polyclonal antibodies. Results: DNA sequencing of PCR products revealed that AQP2 and AQP3 mRNA as well as AQP7 and AQP8 are expressed in human and rat testes. In human and rat testes, AQP2 are expressed in spermatozoa interstitial dells and myofibroblasts and AQP3 are expressed in myofibroblasts of semineferous tubules on immunocytochemical stain. Conclusion: These results indicate that multiple aquaporins are expressed in testes, and that they may have important roles in the spermatogenesis and the germ cell function of testis.
키워드 : Aquaporin, Molecular, Spermatogenesis, Testis
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