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대한생식의학회지 제27권 제2호 2010년
사람 난소조직의 초자화 냉동보존과 면역결핍 동물에의 이식
차병원 여성의학연구소1, 포천중문 의과대학교 생리학교실2, 산부인과학교실3
이경아1,2, 윤세진1, 이숙현1, 신창숙1, 최안나3, 조용선1,3, 윤태기1,3, 차광렬1,3,
Xenografting of the Human Vitrified Ovarian Tissues into the Immune Deficient Animal
Kyung-Ah Lee1,2, Se-Jin Yoon1, Sook Hyun Lee1, Chang Sook Shin1, Anna Choi3, Yong Seon Cho1,3, Tae-Ki Yoon1,3, Kwang Yul Cha1,3
Infertility Medical Center, CHA General Hospital1, Department of Physiology2, Department of Obstetrics and Gynecology3, College of Medicine, Pochon CHA University, Seoul 135-081, Korea
Objective: The present study was conducted to evaluate the viability of germ cells from the adult and fetal ovarian tissues after vitrification followed by xenografting. Method: The human adult ovarian tissues were obtained from 33 years old patient, and the fetal ovarian tissues were obtained from 22 weeks and 25 weeks in gestation. Ovarian tissues were cryopreserved by vitrification with 5.5 M ethylene glycol (EG 5.5) and 1.0 M sucrose as cryoprotectants. Adult and fetal ovarian tissues were pre-equilibrated with EG 5.5 at room temperature for 10 and 5 minutes, respectively and plunged into liquid nitrogen immediately. Frozen-thawed tissues were xenografted into NOD-SCID mice to evaluate the viability and capacity for further growth of the primordial follicles. Grafts were recovered from the recipients 4 weeks after transplantation and histological analysis was accomplished. Result and Conclusion: Grafts recovered 4 weeks after transplantation contained less number of oocytes and primordial follicles compared to that of the fresh tissues. Survived follicles were mainly primordial and intermediary with larger diameter and more granulose cells. It is confirmed that 1) the ovarian tissues were healthy and the germ cells were survived after vitrification, and 2) the survived fetal primordial follicles after vitrification resumed the growth in the xenografts.
키워드 : Human, Ovarian tissue banking, Vitrification, Xenograft
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