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대한생식의학회지 제27권 제2호 2010년
난자채취 2일과 5일에 연속으로 실시한 배아이식의 안전성과 효과
경북대학교벼원 산부인과학교실1, 대구대학교 축산학과2
박기상1,2, 송해범2, 이택후1, 전상식1,
Subsequent Embryo Transfers (SET) on Day 2 and Day 5: It’s Safety and Effectiveness
Kee Sang Park1,2, Hai Bum Song2, Taek Hoo Lee1, Sang Sik Chun1
1Department of Obstetrics and Gynecology, Kyungpook National University Hospital, Taegu, 2Department of Animal Science, Teagu University, Kyungbuk, Korea
Objective: In vitro fertilization (IVF) and a prolonging the time of culture may be helpful in establishing a viable pregnancy through a selection effect. Some embryos do not develop beyond the 4-cell stage and some may not develop to the blastocyst stage. We have evaluated the safety of SET and the outcomes of pregnancy. Methods: Sperms were treated with Ham’s F-10 supplemented with 10% human follicular fluid (hFF). Oocytes of fertilized oocytes were cultured in Dulbecco’s Modified Eagle Medium (DMEM) with 10% of 20% hFF respectively. Up to five oocytes were inseminated with approximately 200,000 sperm cells/2 ml in each well. Fertilization was examined in the following morning and fertilized oocytes were co-cultured until embryo transfer. Vero cells for co-culture were prepared in Tissue Culture Medium – 199 (TCM-199) with 10% fetal bovine serum. At the two to four cell and blastocyst on day 2 and day 5, embryo and blastocyst grading were evaluated. Pregnancy rate was determined after transfer of human embryos at the two to four cell stage on day 2 (Group I) or subsequent transfer of embryos on day 2 and at the blastocyst stage on day 5 (Group II). For statistical analysis, Student’s t-test and Chi-square (x2-test) were used. Results were considered statistically significant when p value was less than 0.05. Results: No differences was found in the fertilization between Group I (81.0%, 98/121) and Group II (81.8%, 180/220). In case of cleavage rate, no difference was found in Group I (95.9%, 94/98) and Group II (97.8%, 174/178). However, the rate of clinical pregnancy was significantly higher (p=0.014) in Group II (66.7%, 12/18) than in Group I (26.3%, 5/19). Conclusion: The results of this study showed that SET is safe and effective, and significantly increases the pregnancy rate.
키워드 : In vitro fertilization (IVF), Subsequent embryo transfer (SET), Clinical pregnancy
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