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대한생식의학회지 제27권 제2호 2010년
Fertilization Promoting Peptide가 사람 정자의 운동양태, 수정능력획득 및 첨체반응에 미치는 영향
한양대학교 자연과학대학 생명과학과1, 을지병원 의과학연구소2, 을지대학교 의과대학 비뇨기과교실3, 생리학교실4
강희규1,2, 김묘경2, 김동훈2, 한성원2, 최도연3, 이호준2,4, 김문규1,
Effect of Fertilization Promoting Peptide on Kinematic Parameters, Capacitation and Acrosome Reaction in Human Spermatozoa
Hee-Gyoo Kang1,2, Myo Kyung Kim2, Dong Hoon Kim2, Sung Won Han2, Do Hyung Choi3, Ho Joon Lee2,4, Moon Kyoo Kim1
1Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133-791; 2Eulji Medical Science Institute, Eulji Medical Center, Seoul 139-711; 3Department of Urology, and 4Physiology, School of Medicine, Eulji University, Taejon 301-726, Korea
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Objective: This study has been carried out to evaluation the effect of fertilization promoting peptide (FPP) on the kinematic parameters, capacitation and acrosome reaction of the frozen-thawed human spermatozoa. Methods: After FPP treatment, we examined kinematic parameters, capacitation and acrosome reaction, usng the methods of computer-aided sperm analysis (CASA) and chlortetracycline (CTC) fluorescence analysis. Results: We have obtained the evidence that FPP can promote the capacitaton and inhibit the spontaneous acrosome reaction of frozen-thawed human spermatozoa in vitro. FPP (25~100 nM) induced a significant increase in the proportion of B-pattern capacitated spermatozoa, and a significant decrease in the proportion of F-pattern uncapacitated ones without significant stimulation of acrosomal exocytosis. In the kinematic parameters treatment, FPP treated groups maintained higher LIN, BCF and STR than those of control. The VAP, VSL, VCL and ALH were not different. Therefore it is suggested that FPP in human seminal plasma may play a positive role in promoting human sperm function.
키워드 : Fertilization promothing peptide, Acrosome reaction, Capacitation, Kinematic parameter, Human spermatozoa
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