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대한생식의학회지 제27권 제4호 2010년
반응성 산소족이 사람 정자의 운동성에 미치는 영향
을지의과대학, 을지병원 의과학연구소1, 생리학교실2, 을지대학병원 산부인과3, 한양대학교 생명과학과4
강희규1, 김동훈1, 한성원1, 김묘경1, 권혁찬3, 이호준1,2, 김문규4,
Effects of Reactive Oxygen Species on Motility in Human Spermatozoa
Hee Gyoo Kang1, Dong Hoon Kim1, Sung Won Han1, Myo Kyung Kim1, Hyuck Chan Kwon3, Ho Joon Lee1,2, Moon Kyoo Kim4
Euji Medical Science Institute, Eulji Medical Center, Seoul 139-711 1, Department of Physiology2 and OB/GYN, School of Medicine, Eulji University, Taejon 301-726 3, 한양대학교 Department of Life Science, College of Natural Science, Hanyang University, Seoul 133-791 4
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Objective: To investigate the effects of ROS on kinematic parameters in Human spermatozoa. To verify the changes in above parameters, Human spermatozoa were incubated with xanthine-xanthine oxidase (X-XO), H2O2, sodium nitroprusside (SNP) or lymphocyte. Otherwise, spermatozoa were incubated under low O2 (5%) condition. Methods: CASA was employed to analyze sperm motion parameters. Results: Under H2O2 treatment, all kinematic parameters of spermatozoa were dramatically increased during 30 min, but gradually decreased thereafter. Under the low concentration of H2O2 (125 uM and 250 uM), the movement velocity (VAP, VCL, VSL) decreased, but forward movement increased. Under the 1 mM H2O2, sperm showed reduced kinematic parameters except during first 30 min of incubation. In the cases of X-XO and SNP treatment, the movement velocity increased but the forward movement reduced. After incubation for 3 hr treatment, the kinematic parameters gradually decreased in high concentration of X-XO. However these parameters maintained or increased in low concentration of X-XO. There was no obvious changes in the above parameters decreased, but forward movement decreased. Taken togethers, it suggested that ROS increased the movement velocity but decreased the forward movement and lateral head replacement. H2O2, X-XO, SNP and lymphocyte treatment significantly increased capacitated spermatozoa within 1 h of incubation. There was no significant difference in capacitation between low- and high o2 group. Conclusion: The early onset of capacitation in the presence of ROS suggest that ROS might be a positive regulator of sperm capacitation and hyperactivation. These results demonstrate that low concentration ROS Facilitates the movement velocity but high concentration ROS was inhibitory.
키워드 : Reactive oxygen species, CASA, Motility, Sperm
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