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대한생식의학회지 제13권 제1호 2010년
돼지 난소내 여포의 폐쇄과정중 과립세포의 결합능의 변화
김천보건전문대학 임상병리과;한양대학교 자연과학대학 생물학과;한양대학교 자연과학대학 생물학과;한양대학교 자연과학대학 생물학과;
장철수;이창주;윤용달;김문규;,
Change of hCG Binding Capacity on the Granulosa Cell of Porcine Ovary During Follicular Atresia
Chang, Choul-Soo;Lee, Chang-Joo;Yoon, Yong-DaI;Kim, Moon-Kyoo;
;Department of Biology, College of Natural Sciences, Hanyang University;Department of Biology, College of Natural Sciences, Hanyang University;Department of Biology, College of Natural Sciences, Hanyang University;
In order to study the mechanism of follicular atresia, follicles were classified into the normal groups and the atretic ones, according to the criteria with or without corpus luteum, size of follicles, vascularization, status of granulose cells and the hypertrophy of theca layers in the porcine ovary. To estimate the binding capacity of human chorionic gonadotropin (hCG) receptor on the granulosa cells during atresia, hCG were iodinated by the chloramine-T method and then purified through the column chromatography. The concentration" of hCG receptor in each group were measured by the hCG receptor binding assay. Binding capacity in large normal follicles were 1.16%, but atretic ones were 0.45%. But in medium and small follicles (below 6mm in diameter), the binding capacity in normal follicles were 0.09%, but atretic ones were 0.05%, which was lower than those of large follicles. The present ( ) that the concentrations of hCG receptors on granulosa cells is decreased when the follicles become atretic and be used as a sort of creteria for the identification of follicles atresia.
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