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대한생식의학회지 제18권 제1호 2010년

생쥐의 정자 추출물이 정자-난자의 결합에 미치는 영향

한양대학교 자연과학대학 생물학과;한양대학교 자연과학대학 생물학과;제일병원 체외수정 연구실;한양대학교 자연과학대학 생물학과;한양대학교 자연과학대학 생물학과;

김문규;계명찬;최규완;윤현수;김종흡;,

Effects of Sperm Extracts on Sperm - Egg Binding in Mouse

Kim, Moon-Kyoo;Gye, Myung-Chan;Choi, Kyoo-Wan;Yoon, Hyun-Soo;Kim, Jong-Heup;

Department of Biology, College of Natural Sciences, Hanyang University;Department of Biology, College of Natural Sciences, Hanyang University;IVF Research Laboratory, Cheil General Hospital;Department of Biology, College of Natural Sciences, Hanyang University;Department of Biology, College of Natural Sciences, Hanyang University;

In order to study the sperm-egg interaction during fertilization process in mouse, the effects of sperm concentration, the duration of capacitation and insemination, the stages of maturation and development of eggs, and sperm extracts and BSA on sperm binding to egg were examined. Sperm-egg binding was increased depending on sperm concentration within the range of $10^3-10^6$ sperm/ml. It showed the most numbers of sperm-egg binding at 60min from the beginning of preincubation(capacitation) and insemination, respectively. During sperm capacitation, sperm-egg binding inhibitor was released from sperm into the incubation medium. Sperm extracts containing trypsin-like enzyme which is secreted through the acrosome reaction increased the binding. BSA in the culture medium showed a positive effect on the binding. It is suggested that physicochemical alterations of zona pellucida in the process of maturation and fertilization of eggs leaded to inhibition of sperm-egg binding.

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