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대한생식의학회지 제18권 제1호 2010년

생쥐난자의 성숙단계와 결빙억제제에 따른 동결 및 해빙 후 생존율에 관한 연구

제일병원 체외수정 연구실;제일병원 체외수정 연구실;한양대학교 생물학과;한양대학교 생물학과;한양대학교 생물학과;

최규완;이호준;강희규;전용필;김문규;,

Study on the Survival of Frozen-Thawed Mouse Oocytes According to Maturation Stage and Cryoprotectants

Choi, Kyoo-Wan;Lee, Ho-Joon;Kang, Hee-Kyoo;Chun, Yong-Pil;Kim, Moon-Kyoo;

IVF Research Laboratory, Department of Obstetrics and Gynecology, Cheil General Hospital;IVF Research Laboratory, Department of Obstetrics and Gynecology, Cheil General Hospital;Department of Biology, College of Natural Sciences, Hanyang University;Department of Biology, College of Natural Sciences, Hanyang University;Department of Biology, College of Natural Sciences, Hanyang University;

In order to increase the pregnancy rate by means of cryopreservation of the excess oocytes in IVF-ET program, the survival rate of the frozen-thawed oocytes of mouse was examined according to the stages of maturation, cryoprotectants and their treatment. The results were summarized as follows. First, during the continuous treatment with cryoprotectant media, the survival rate of oocytes was higher in DMSO than in PROH, and higher at low temperature($4^{\circ}C$) than at room temperature($25^{\circ}C$). Second, as regard with the maturation of immature(GV-intact) oocytes after treatment with cryoprotectant media, the rate of maturation in DMSO-treated group(52%) was higher than in PROH-treated group(35%). Third, according to the treatment of cryoprotectant media, the survival rate of frozen-thawed oocytes in DMSO-treated group (45%) was higher than in PROH-treated group(29%), and that of oocytes in DMSO 4-step treated group was higher than any other groups. Finally, in the post-thaw oocytes frozen at various stage of maturation, the survival rate of immature oocytes with GV was the highest in all groups. These results suggest that in the cryopreservation of mouse oocytes, DMSO was better than PROH as cryoprotectant, in treatment of cryprotectant the multi-step treatment was better than single-step, and the post-thaw survival rate of oocytes was closely related to the maturity of oocytes. It is assumed that the highest survival rate of mouse oocytes with GV is due to the stability of the structures in nucleus and intracelluar organelles, and of physiological function.

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