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대한생식의학회지 제18권 제1호 2010년

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경희대학교 의과대학 산부인과학교실 (불임크리??);경희대학교 의과대학 산부인과학교실 (불임크리??);경희대학교 의과대학 산부인과학교실 (불임크리??);건국대학교 축산학과;건국대학교 축산학과;

김재명;서병희;이재현;유승환;정길생;,

Cryopreservation of Hamster Oocytes and its Clinical Uses

Kim, Jae-Myeoung;Suh, Byung-Hee;Lee, Jae-Hyun;Yu, Seung-Hwan;Chung, Kil-Sheng;

Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University;Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University;Infertility Clinic, Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University;Department of Animal Husbandary, Kon Kuk University;Department of Animal Husbandary, Kon Kuk University;

There studies were carried for evaluation of the efficiency of freezing of hamster oocytes for use in a human sperm penetration assay. The hamster oocytes fully equilibrated in various cryoprotectant agents and inseminated with human sperm. After insemination with hamster oocytes, there was no difference in penetrated rates. Cumulus free oocytes equilibrated in 1.5M various cryoprotective agents and slowely cooled to temperature $-30^{\circ}C$ before rapid cooling and storage in nitrozen tank. After rapid thawing, survival rates of frozen oocytes according to cryo-protective agents were examined and the human sperm penetration assay with zona free hamster oocytes was conducted. 1. Survival rates of oocytes after cryoprotectants exposure have no significant difference (range 88-91%) and peneration rate was 51.1%. 2. Recovery and survival rate of frozen-thawed oocytes were 85.1 and 66.8%. There was no significant difference on cryoprotective agents. 3. Penetration rates of the frozen-thawed and intact oocytes were 69.0 and 77.0%, respectively. 4. Hamster oocytes cryopreservation provides a convenient way of supplying and trans-porting hamster oocytes for the assessment of the fertilizing potential of human spermatozoa.

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