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대한생식의학회지   제18권 제1호 2010년

정자 준비에서의 Wang's Tube 효과에 관한 연구

고려대학교 의과대학 산부인과학교실;고려대학교 의과대학 산부인과학교실;고려대학교 의과대학 산부인과학교실;고려대학교 의과대학 산부인과학교실;고려대학교 의과대학 산부인과학교실;고려대학교 의과대학 산부인과학교실;

김영태;김용욱;김해중;김선행;나중열;구병삼;,

A Study of Effects of Wang's Tube in Semen Preparation

Kim, Young-Tae;Kim, Yong-Ook;Kim, Hae-Jung;Kim, Sun-Haeng;Rha, Joong-Yol;Ku, Pyong-Sahm;

Department of Obstetrics and Gynecology, College of Medicine, Korea University;Department of Obstetrics and Gynecology, College of Medicine, Korea University;Department of Obstetrics and Gynecology, College of Medicine, Korea University;Department of Obstetrics and Gynecology, College of Medicine, Korea University;Department of Obstetrics and Gynecology, College of Medicine, Korea University;Department of Obstetrics and Gynecology, College of Medicine, Korea University;

Swim-up and Wang's tube system are known methods of motile sperm selection and aerobic bacterial removal from the raw semen. This study was designed to evaluate the recovery rate of motile sperm, % normal morphology of sperm, the efficiency of bacterial removal after sperm preparation by the above two methods. The results were as follows. 1. There was more significant increase of sperm concentration in preparation by swim-up than Wang's tube (p<0.05). The concentration of sperm by swim up was changed from $82.5{\times}10^6/ml$ to $6836{\times}10^6/ml$, and Wang's tube was changed from $82.5{\times}10^6/ml$ to $36.0{\times}10^6/ml$. 2. There was significant increase in sperm motility after preparation by two methods in comparison with initial sperm motility (p<0.05), but no statistical difference between two methods was noted. The % motility of sperm by swim-up was increased from 66.1% to 95.7% and Wang's tube from 66.1% to 98.1%. 3. There was significant increase of % normal morphology of sperm in the samples prepared by two methods (p<0.05), from 49.2% to 85.3% in swim-up and from 49.2% to 92.1% in Wang's tube, but there was no statistical significance between them. 4. There was no bacterial growth in aerobic culture after preparation by two methods.

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