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대한생식의학회지 제18권 제2호 2010년

인간 양수에 의한 생쥐 전핵기 1-세포배의 체외발생 촉진효과에 관한 연구

서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;

정구민;문신용;이진용;장윤석;,

A Study on in Vitro Developmental Promoting Effect of Pronucleate 1-Cell Mouse Embryos by Human Amniotic Fluid

Chung, Ku-Min;Moon, Shin-Yong;Lee, Jin-Yong;Chang, Yoon-Seok;

Department of Obstretrics and Gynecology, College of Medicine, Seoul National University;Department of Obstretrics and Gynecology, College of Medicine, Seoul National University;Department of Obstretrics and Gynecology, College of Medicine, Seoul National University;Department of Obstretrics and Gynecology, College of Medicine, Seoul National University;

The purpose of this study was to evaluate the in vitro developmental promoting effect of human amniotic fluid (AF) on pronucleate 1-cell mouse embryos. The AF was obtained from five patients undergoing amniocentesis for the routine diagnosis of fetal abnormality. The supernatant was filtered ($0.22{\mu}m$) after centrifugation and stored at $-20^{\circ}C$. One-cell embryos were cultured in four study groups (10% AF, 0.4% BSA, 10% AF+0.4% BSA & no-supplement in Ham's F10) for 6 days (EXP. 1). Significantly more embryos hatched in 10% AF (P<0.0l), although no difference was found among other three groups. The embryos were also cultured in varous concentration of AF (0, 10, 50 & 100%) for 7 days (EXP. 2). The rate of hatched blastocysts was significantly higher in 10%- and 50% group than in 0% and 100%- one at day 6 (P<0.05) and day 7 (P<0.005), although no difference was found between 10% and 50%- group.

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