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대한생식의학회지 제19권 제2호 2010년
난관체류시간에 따른 생쥐초기배의 체외발생능력
대구대학교 농과대학 축산학과;대구대학교 농과대학 축산학과;대구대학교 농과대학 축산학과;고려대학교 자연자원대학 축산학과;고려대학교 자연자원대학 축산학과;
송해범;서병부;김광식;박성은;이상호;,
Dependence of Mouse Embryonic Development in vitro on the Exposed Period to Oviductal Environment
Song, H.B.;Seo, B.B.;Kim, K.S.;Park, S.E.;Lee, S.H.;
Department of Animal Science, College of Agriculture, Taegu University;Department of Animal Science, College of Agriculture, Taegu University;Department of Animal Science, College of Agriculture, Taegu University;Department of Animal Science, College of Natural Resources, Korea University;Department of Animal Science, College of Natural Resources, Korea University;
Development in vitro of 2-cell mouse embryos was examined after appropriate exposure to oviductal milieu to demonstrate biological activity present in the oviducts. ICR and ($C57Bl/6{\times}Balb/c$) $F_1$ hybrid mice were superovulated and mated for the recovery of early embryos. Embryos were recoverd at every 2h intervals from 32h post-hCG(hph) to 56 hph. The proportions of developmental stages were determined in the recovered embryos. Development in vitro of 2-cell embryos was more rapid in $F_1$ hybrid than in ICR, showing high proportions of 4-cell embryo and blastocyst at 120 hph. 100% of blastocyst development was obtained at 38hph in $F_1$ hybrid and at 50 hph in ICR when 2-cell embryos were cultured upto 120hph in vitro. Moreover, in vitro culture of oviducts containing 2-cell embryos in ICR mice for 12h from 34hph to 46hph increased developmental capacity of ICR mouse embryo in vitro. The results indicate that oviductal environment contains substances having mitogenic activity and overcoming early cell block in vitro. The mitogenic activity is effective in vitro as well as in vivo.
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