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대한생식의학회지 제19권 제2호 2010년
인간의 미성숙난자의 동결보존에 관한 연구
차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;
김은경;손원영;지희준;고정재;윤태기;차광열;,
Cryopreservation of Human Immature Follicular Oocyte
Kim, Eun-Kyung;Son, Weon-Young;Chi, Hee-June;Ko, Jung-Jae;Yoon, Tae-Ki;Cha, Kwang-Yul;
Department of Obstetrics and Gynecology, Infertility Medical Center of Cha Women's Hospital;Department of Obstetrics and Gynecology, Infertility Medical Center of Cha Women's Hospital;Department of Obstetrics and Gynecology, Infertility Medical Center of Cha Women's Hospital;Department of Obstetrics and Gynecology, Infertility Medical Center of Cha Women's Hospital;Department of Obstetrics and Gynecology, Infertility Medical Center of Cha Women's Hospital;Department of Obstetrics and Gynecology, Infertility Medical Center of Cha Women's Hospital;
This study was carried out to set up the ovum bank for ovum donation and to determine the best freezing method for human immature oocytes. Human immature follicular oocytes were cryopreserved by slow freezing and rapid thawing method. Immature follicular oocytes were treated by propanediol(PROH) solution by 2 and 4 step method in protocols A & B, respectively. In protocol C, immature oocytes were exposed to sucrose prior to treatment of PROH by 4 step method. We compared survival rate, maturation rate, and fertilization rate of immature oocytes among three protocols. Results were as follows. 1. Oocytes treated by the protocol C showed the highest survival rate( 70.3 %) and maturation rate(34.6%) after thawing. 2. Survival rate of oocytes treated by the protocol C was significantly higher than that of the protocol B after thawing(p<0.05). In conclusion, treatment of oocytes with sucrose prior to expose PROH was the best freezing method. Sucrose may have reduced the toxic effect of cryoprotectant to oocytes. We failed to induce fertilization of oocytes, which were treated by any protocols, by conventional insemination method, but obtained 28.8% fertilization rate by using partial zona dissection(PZD) method. This result suggests that micromanipulation(PZD) of the thawed oocytes before insemination will improve the fertilization rate.
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