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대한생식의학회지 제20권 제2호 2010년
인간정자에 있어서 정자처리법의 비교
서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;중앙대학교 의과대학 산부인과학교실;중앙대학교 산업대학 축산학과;중앙대학교 산업대학 축산학과;
방명걸;정구민;신창재;김정구;문신용;장윤석;이진용;이상훈;정영채;김창근;,
Comparison among the Sperm Preparation Methods on the Human Spermatozoa
Pang, Myung-Geol;Chung, Ku-Min;Shin, Chang-Jae;Kim, Jung-Gu;Moon, Shin-Yong;Chang, Yoon-Seok;Lee, Jin-Yong;Lee, Sang-Hoon;Chung, Yung-Chai;Kim, Chang-Keun;
Department of Obstetrics and Gyecology. College of Medicine. Seoul National University;Department of Obstetrics and Gyecology. College of Medicine. Seoul National University;Department of Obstetrics and Gyecology. College of Medicine. Seoul National University;Department of Obstetrics and Gyecology. College of Medicine. Seoul National University;Department of Obstetrics and Gyecology. College of Medicine. Seoul National University;Department of Obstetrics and Gyecology. College of Medicine. Seoul National University;Department of Obstetrics and Gyecology. College of Medicine. Seoul National University;Department of Obstetrics and Gynecology. College of Medicine. Chung-Ang University;Department of Animal Science, Chung-Ang University;Department of Animal Science, Chung-Ang University;
Procedures to separate motile. normal & motile and acrosome-reacted sperm with high efficiency have clinical application in Assisted Reproductive Technology in terms of increasing the probability of fertilization by a normal sperm and subsequent normal embryonic development. This study evaluated the effects of 10 sperm preparation techniques [Swim-up from a washed pellet (SU). Swim-up from semen (SO). Continuous Percoll Gradients I (PIC). Discontinuous Percoll Gradients I (PID). Continuous Percoll Gradients II(P II C). Discontinuous Percoll Gradients II(P II D), SpermPrep (SFC). Wang's tube (WT). Albumin Gradients (AG), Low temperature capacitation (LTC)] on motility (%), normal morphology (%), motile sperm recovery rate(%). morphologically normal & motile sperm recovery rate (%), true acrosome reaction (%) and fertilizing ability. A P II D proved to be an effective means of separating morphologically normal & motile sperm. Our results indicated the P II D has advantages as compared with other methods in terms of recovery rate. enhancement of motility and normal morphology. And a LTC seems to be an effective means of enhancing the true acrosome reaction and fertilizing ability. These results suggest that the combined method of LTC and P II D for separation of morphologically normal & motile sperm and acrosome reacted sperm may be a useful procedure for intrauterine insemination and in vitro fertilization in the management of male factor infertility as well as for isolation of subpopulation of sperm for basic research.
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