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대한생식의학회지 제21권 제3호 2010년
Subfertile 정자처리에 Modified Mini-Percoll 방법의 효용성에 관한 연구
중앙대학교 의과대학 산부인과학교실;중앙대학교 의과대학 산부인과학교실;중앙대학교 의과대학 산부인과학교실;중앙대학교 의과대학 산부인과학교실;중앙대학교 의과대학 산부인과학교실;
김희선;장현정;이희옥;이숙현;이상훈;,
Study on the Effectiveness of Modified Mini-Percoll Method in Subfertile Sperm
Kim, Hee-Sun;Chang, Hyun-Jung;Lee, Hee-Ok;Lee, Sook-Hyun;Lee, Sang-Hoon;
Department of Obstetrics and Gynecology, College of Medicine, Chung-Ang University;Department of Obstetrics and Gynecology, College of Medicine, Chung-Ang University;Department of Obstetrics and Gynecology, College of Medicine, Chung-Ang University;Department of Obstetrics and Gynecology, College of Medicine, Chung-Ang University;Department of Obstetrics and Gynecology, College of Medicine, Chung-Ang University;
Semen preparation for Assisted Reproductive Technology(ART) involves the separation of the motile sperm from non-motile, dead sperm, cellular debris and seminal fluid. The aim of this study is to compare the motile sperm recovery rate and motility index of different sperm preparation method(swim-up method, 80% isotonic continuous percoll method, two-layer discontinuous percoll method, mini-percoll method). In Mini-Percoll method, pellets from patients were suspended in 0.3ml of medium and layerd on a discontinuous percoll gradient consisting of 0.3ml each of 50, 70, 95% isotonic percoll. All semen samples are divided into normal and subnormal sperm group(oligo-, astheno-, oligoasthenozoospermia). Especially, we evaluated the effect of mini-percoll method in subnormal sperm group. In normal sperm group, mini-percoll method and two layer discontinuous percoll method (40%/80%) allowed increasing of motile sperm recovery rate. But motility index was higher in swimup method than the other methods. In subnormal sperm group, mini-percoll method has advantages as compare with the other methods in motile sperm recovery rate and motility index. These results suggest that modified mini-percoll method could be certainly a valuable tool in some cases of severe male factor sperm.
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