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대한생식의학회지 제21권 제3호 2010년
생쥐 초기배아의 발생시기와 냉동보존 방법에 따른 발생률
한양대학교 자연과학대학 생물학과;제일병원 체외수정연구실;한양대학교 자연과학대학 생물학과;
전용필;이호준;김문규;,
Development Rates of the Cryopreserved Mouse Embryos According to the Embryonic Stage and Cryopreservation Method
Cheon, Yong-Pil;Lee, Ho-Jun;Kim, Moon-Kyoo;
Dmepartent of Biology, College of Natural Sciences, Hanyang University;IVF Research Laboratory, Cheil General Hospital;Dmepartent of Biology, College of Natural Sciences, Hanyang University;
The study has been carried out in order to evaluate the effects of embryonic stage, and cryopreservation method on the rates of viability and development of the cryopreserved mouse early embryos. The results were as following:In the treatment steps of cryoprotectant, for the fertilized oocyte with pronucleus(PN), 2-step was better than the others. And for the other embryos, 4-step was better than 2- or 3-step. In respect to the embryonic stage, as the embryos developed from fertilized oocytes to 8-cell embryos, the rates of viability and development were increased higher. Therefore, 8-cell embryo was better stage than the others. In respect to the kind of cryoprotectants, PROH was better than DMSO for the fertilized oocyte, as a cryoprotectant. DMSO, for the 2-cell embryos and PROH and DMSO for the 4- and 8-cell embryos were suitable for cryopreservation.
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