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대한생식의학회지 제22권 제3호 2010년
세포질내 정자주입을 시행한 인간 난자의 전핵 형성 및 배아 발생에 관한 연구
제일병원 불임연구실;제일병원 불임연구실;제일병원 불임연구실;제일병원 불임연구실;한양대학교 자연과학대학 생물학과;한양대학교 자연과학대학 생물학과;
전진현;임천규;박용석;이호준;김종흡;김문규;,
Pronucleus Formation and Embryonic Development of the Human Oocytes Fertilized by Intracytoplasmic Sperm Injection
Jun, Jin-Hyun;Lim, Chun-Kyu;Park, Yong-Seog;Lee, Ho-Joon;Kim, Jong-Heup;Kim, Moon-Kyoo;
Infertility Research Laboratory, Cheil General Hospital;Infertility Research Laboratory, Cheil General Hospital;Infertility Research Laboratory, Cheil General Hospital;Infertility Research Laboratory, Cheil General Hospital;Department of Biology, College of Natural Sciences, Hanyang University;Department of Biology, College of Natural Sciences, Hanyang University;
Mammalian, including human, spermatozoa undergo morphological and physiological changes during sperm maturation. There were, these changes may affect the fertilization and embryonic development. In this study, we examined the pronucleus formation, pronucleus disappearance and embryonic development in the human oocytes fertilized by intracytoplasmic sperm injection (ICSI). The injected spermatozoa were grouped into ejaculated, epididymal and testicular by the collecting region. Among 363 metaphase II injected oocytes, 287(79.1%) oocytes were normally fertilized and displayed two pronuclei. There were no difference in the fertilization rates and in the pronucleus formation and pronucleus disappearance at 16, 20 and 24 hr after ICSI, among the each spermatozoa group. Also, at 64 hr, the appearance of embryonic development was similar. From these results it can be concluded that there was no difference of maturity among the sperm collected from ejaculated, epididymis and testis in the pronucleus formation and embryonic development. Therefore, testicular spermatozoa are successfully used with ICSI in IVF-ET program.
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