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대한생식의학회지 제22권 제3호 2010년

사람 다수정난자의 체외배양시 Fragmented Embryo와 Non-fragmented Embryo에서의 Methionine 유입량 및 미토콘드리아 분포양상의 비교

차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;

도병록;정미경;장미경;이경아;고정재;윤태기;차광열;,

Mitochondrial Distribution and Methionine Uptake in Fragmented and Non-fragmented Embryos Derived from Multi-pronuclei Zygotes in Human In Vitro Fertilization (IVF) Program

Do, B.R.;Chung, M.K.;Chang, M.K.;Lee, K.A.;Ko, J.J.;Yoon, T.K.;Cha, K.Y.;

Infertility Medical Center, CHA General Hospital;Infertility Medical Center, CHA General Hospital;Infertility Medical Center, CHA General Hospital;Infertility Medical Center, CHA General Hospital;Infertility Medical Center, CHA General Hospital;Infertility Medical Center, CHA General Hospital;Infertility Medical Center, CHA General Hospital;

Despite the frequent incidence of embryo fragmentation in early human embryos, the reason of the embryo fragmentation has not been known yet. This study was conducted to investigate the histological difference(s) between fragmented (FR) and non-fragmented (NFR) human embryos focusing on comparison of mitochondrial distribution and protein synthesis. Multi-pronuclei zygotes (MPZ) such as three or more pronuclei containing in human in vitro fertilization and embryo transfer (IVF-ET) program were used for this study. MPZ were cultured in TCM-199 supplemented with 10% of human fetal cord serum (hFCS) in 5% $CO_2$ incubator at $37^{\circ}C$ for 24 hours. The cleaved embryos to 2-4 cells after 24 hours were grouped by their grade of fragmentation. Embryos were stained with Rhodamine123 (Rh123) and fluorescence was evaluated under the fluorescence microscope through PB 450-490 filter (Leitz). Regarding to protein synthesis during early human embryogenesis, there is no significant difference in the amount of synthetic proteins between FR and NFR embryos. Distribution of cytoplasmic organelles in embryos was evaluated by transmission electron microscope (TEM). The cytoplasmic distribution of mitochondria was different between FR and NFR embryos. The mitochondrial distribution was even in NFR, whereas severely aggregated in FR. It is not able to clarify in the present study whether this uneven mitochondrial distribution in FR embryo is the reason for embryo fragmentation or is the result from fragmentation. Physiological disparity related to the mitochondrial distribution may be one of the reasons for embryo fragmentation. Further studies should be addressed to investigate the physiological differences between FR and NFR embryos.

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