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대한생식의학회지 제23권 제1호 2010년
생식보조시술시 단백질원으로서 인간난포액의 적합성 및 효율성에 관한 연구;II. 인간난포액이 생쥐수정란의 체외발달에 미치는 효과
한나여성의원 시험관아기센터;한나여성의원 시험관아기센터;한나여성의원 시험관아기센터;한나여성의원 시험관아기센터;한나여성의원 시험관아기센터;건국대학교 동물자원연구센터;
김동훈;지희준;김지연;구정진;장상식;정길생;,
Studies on the Suitability and Efficiency of Human Follicular Fluid as Protein Supplement in Assisted Reproductive Technology(ART);II. Effect of Human Follicular Fluid on Development of Mouse Embryos In Vitro
Kim, D.H.;Chi, H.J.;Kim, J.Y.;Koo, J.J.;Chang, S.S.;Chung, K.S.;
IVF Center, Hanna Women's Clinic;IVF Center, Hanna Women's Clinic;IVF Center, Hanna Women's Clinic;IVF Center, Hanna Women's Clinic;IVF Center, Hanna Women's Clinic;Animal Resources Research Center, Kon-Kuk University;
This study was performed to investigate the effect of human follicular fluid (HFF) on development of mouse embryos, for evaluating the suitability of HFF as a substitutive material of human fetal cord serum in ART program. The various concentrations of HFF were added into the culture medium and the effects of HFF concentrations were examined to identify the optimal concentration of HFF for embryo development. The potency of HFF in improving embryo development was compared to that of other protein supplement. Collected HFFs were classified with the maturity of the containing oocytes; mature, immature, atretic, and then the effects of the classified HFFs on embryo development were examined. Also, HFF was separated into the low (<30,000 Da) and high (>30,000 Da) molecular weight fractions and the effects of the fractions on embryo development were investigated. The highest development rate was found in culture medium supplemented with 20% HFF, bnt this rate was reversely reduced at the concentrations of HFF higher than 20%. The development rates to the blastocyst, hatching blastocyst, attachment and outgrowth cultured in mature HFF was significantly higher than those in immature and atretic HFF, and mean cell number in blastocyst was higher in mature HFF than in immature and atretic HFF. The development rates of mouse embryos according to protein sources were significantly higher in HFF than in fetal cord serum (FCS), maternal serum (MS) and bovine serum albumin (BSA), and mean cell number in blastocyst cultured in HFF was higher than that in FCS, MS and BSA. The development rates of embryo and mean cell number in blastocyst cultured in high molecular weight fraction of HFF were higher than those in low molecular weight fraction, but the results of high molecular weight fraction were lower than those of whole HFF. Therefore, these results indicated that human mature follicular fluid was useful for improving the development of mouse embryos, which suggests a possibility that HFF also may be used efficiently for improving the culture condition in human ART program as a protein supplement.
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