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대한생식의학회지 제24권 제3호 2010년
국산 Fluorescence in Situ Hybridization 시스템을 이용한 다양한 검체에서의 염색체 분석
서울대학교 의과대학 산부인과학교실;서울대학교 의학연구원 인구의학연구소;서울대학교 의학연구원 인구의학연구소;서울대학교 의학연구원 인구의학연구소;함춘여성크리닉;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;서울대학교 정밀기계설계공동연구소;바이오메드 랩;서울대학교 의과대학 산부인과학교실;서울대학교 의과대학 산부인과학교실;
문신용;방명걸;오선경;류범용;황도영;정병준;최진;손철;장준근;김종원;김석현;최영민;,
Chromosome Analysis in Clinical Samples by Chromosome Diagnostic System Using Fluorescence in Situ Hybridization
Moon, Shin-Yong;Pang, Myung-Geol;Oh, Sun-Kyung;Ryu, Buom-Yong;Hwang, Do-Yeong;Jung, Byeong-Jun;Choe, Jin;Sohn, Cherl;Chang, Jun-Keun;Kim, Jong-Won;Kim, Seok-Hyun;Choi, Young-Min;
Department of Obstetrics and Gynecology, College of Medicine, Seoul National University;Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University;Ham Choom Women's Clinic;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University;Institute of Advanced Machinery and Design, Seoul National University;Biomed Lab;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University;Department of Obstetrics and Gynecology, College of Medicine, Seoul National University;
Fluorescence in situ hybridization (FISH) techniques allow the enumeration of chromosome abnormalities and from a great potential for many clinical applications. In order to produce quantitative and reproducible results, expensive tools such as a cooled CCD camera and a computer software are required. We have developed a Chromosome Image Processing System (Chips) using FISH that allows the detection and mapping of the genetic aberrations. The aim of our study, therefore, is to evaluate the capabilities of our original system using a black-and-white video camera. As a model system, three repetitive DNA probes (D18Z1, DXZ1, and DYZ3) were hybridized to variety different clinical samples such as human metaphase spreads and interphase nuclei obtained from uncultured peripheral blood lymphocytes, uncultured amniocytes, and germ cells. The visualization of the FISH signals was performed using our system for image acquisition and pseudocoloring. FISH images were obtained by combining images from each of probes and DAPI counterstain captured separately. Using our original system, the aberrations of single or multiple chromosomes in a single hybridization experiment using chromosomes and interphase nuclei from a variety of cell types, including lymphocytes, amniocytes, sperm, and biopsied blastomeres, were enabled to evaluate. There were no differences in the image quality in accordance with FISH method, fluorochrome types, or different clinical samples. Always bright signals were detected using our system. Our system also yielded constant results. Our Chips would permit a level of performance of FISH analysis on metaphase chromosomes and interphase nuclei with unparalleled capabilities. Thus, it would be useful for clinical purposes.
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