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대한생식의학회지 제24권 제3호 2010년
항정자항체가 일반적 체외수정 방법 및 정자직접 주입법(ICSI)에 미치는 영향에 관한 연구
차병원 여성의학 연구소, 건국대학교 동물자원 연구센터;차병원 여성의학 연구소, 포천중문의과대학교;차병원 여성의학 연구소, 포천중문의과대학교;차병원 여성의학 연구소;차병원 여성의학 연구소, 포천중문의과대학교;차병원 여성의학 연구소, 포천중문의과대학교;건국대학교 동물자원 연구센터;
오종훈;엄기붕;최동희;정미경;한세열;차광열;정길생;,
The Effect of Anti-Sperm Antibodies on Conventional IVF and Intracytoplasmic Sperm Injection (ICSI)
Oh, Jong-Hoon;Oum, Ki-Boong;Choi, Dong-Hee;Chung, Mi-Kyung;Han, Sei-Yul;Cha, Kwang-Yul;Chung, Kil-Saeng;
Infertility Medical Center/Cha General Hospital of Seoul, Animal Resources Research Center, Kon-Kuk University;Infertility Medical Center/Cha General Hospital of Seoul, College of Medicine, Pochon Cha University;Infertility Medical Center/Cha General Hospital of Seoul, College of Medicine, Pochon Cha University;Infertility Medical Center/Cha General Hospital of Seoul;Infertility Medical Center/Cha General Hospital of Seoul, College of Medicine, Pochon Cha University;Infertility Medical Center/Cha General Hospital of Seoul, College of Medicine, Pochon Cha University;Animal Resources Research Center, Kon-Kuk University;
The purpose of this study was to examine the effects of anti-sperm antibody (ASA) on the fertilization processes using conventional IVF and ICSI procedure in human and hamster oocytes. In human IVF, we have observed restricted fertilization with sperm testing positive for ASA. ($23{\sim}90%$ IgA, 60-97 % IgG). However, if ICSI was perform in the next IVF cycle with the same patients, we could successfully fertilize the oocytes (37%; p<0.001), thus achieving pregnancy and delivery. When the sperm were cocultured in medium containing ASA, there were binding of ASA to sperm surface. In addition, the mean rate of the acrosomal reaction in an in vitro acrosome reaction test was lower for Ab-bound sperm (43.5%) than for Ab-free sperm group (51.3%, p<0.05). We used human sperm and hamster oocytes to confirm the negative effects of the ASA on fertilization. The sperm and/or oocytes have been expose to medium containing ASA before IVF and ICSI. In this experiment, the ASA was bound to the oocyte and sperm surface. The following results were obtain by using various combinations of ASA free or ASA bound sperm with ASA free or ASA bound oocytes for IVF. When ASA free sperm were inseminate with ASA free and ASA bound hamster oocytes, the fertilization rates are 89.6% and 74.3% respectively. However, when ASA bound human sperm were use the results were 62.5% and 55.6% respectively. These shows the fertilization rate was significantly decreased in both ASA bound and ASA free oocytes when using ASA bound sperm. No difference found when ASA are present on the oocyte surface. When the hamster oocytes was treated by ICSI with ASA free or ASA bound human spermatozoa, no significant difference was found. These results showed that ICSI is the most promising method for couples who fertilization was not possible by conventional IVF because of ASA.
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