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대한생식의학회지 제27권 제1호 2010년
인간 난소의 과립 세포 배양 중 Leptin이 스테로이드 생성에 미치는 영향
연세대학교 의과대학 산부인과학교실;아주대학교 의과대학 산부인과학교실;아주대학교 의과대학 산부인과학교실;아주대학교 의과대학 산부인과학교실;연세대학교 의과대학 산부인과학교실;
김세광;김명신;황경주;권혁찬;조동제;,
Effect of Leptin on the Steroidogenesis of Cultured Human Granulosa Cells
Kim, Sei-Kwang;Kim, Myong-Shin;Hwang, Kyung-Joo;Kwon, Hyuck-Chan;Cho, Dong-Jae;
Department of Obstetrics and Gynecology, Yonsei University College of Medicine;Department of Obstetrics and Gynecology, Ajou University School of Medicine;Department of Obstetrics and Gynecology, Ajou University School of Medicine;Department of Obstetrics and Gynecology, Ajou University School of Medicine;Department of Obstetrics and Gynecology, Yonsei University College of Medicine;
Objective: To elucidate the location of leptin and receptors of ovary specimens obtained from patients undergoing hysterectomy by immunohistochemical staining and to determine the effect of leptin on the steroidogenesis of cultured granulosa cells. Method: In the culturing process of the granulosa cells, FSH (1 IU/ml)and leptin (50 ng/ml), IGF-I (50 ng/ml) was administered to each study group (Group I: FSH; Group II: FSH, leptin; Group III: FSH, IGF-I; Group IV: FSH, IGF-I, leptin), and the levels of estradiol, progesterone, androstenedione in the culture media was measured by radioimmunoassay. Statistical analysis was conducted by one-way ANOVA with Scheffe test. Results: The results showed that leptin and leptin receptors were both found to be strongly stained in granulosa and theca cells, and also in some interstitial cells. Leptin receptors were also observed in cultured granulosa cells. While there was no statistically significant difference in the androstnedione concentrations between the groups, estradiol concentrations was significantly decreased in Group IV (
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