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대한생식의학회지 제27권 제2호 2010년
사람 난소조직의 초자화 냉동보존과 면역결핍 동물에의 이식
차병원 여성의학연구소, 포천중문 의과대학교 생리학교실;차병원 여성의학연구소;차병원 여성의학연구소;차병원 여성의학연구소;포천중문 의과대학교 산부인과학교실;차병원 여성의학연구소, 포천중문 의과대학교 산부인과학교실;차병원 여성의학연구소, 포천중문 의과대학교 산부인과학교실;차병원 여성의학연구소, 포천중문 의과대학교 산부인과학교실;
이경아;윤세진;이숙현;신창숙;최안나;조용선;윤태기;차광렬;,
Xenografting of the Human Vitrified Ovarian Tissues into the Immune Deficient Animal
Lee, Kyung-Ah;Yoon, Se-Jin;Lee, Sook-Hyun;Shin, Chang-Sook;Choi, An-Na;Cho, Yong-Seon;Yoon, Tae-Ki;Cha, Kwang-Yul;
Infertility Medical Center, CHA General Hospital, Department of Physiology, Pochon CHA University;Infertility Medical Center, CHA General Hospital;Infertility Medical Center, CHA General Hospital;Infertility Medical Center, CHA General Hospital;Department of Obstetrics and Gynecology, College of Medicine, Pochon CHA University;Infertility Medical Center, CHA General Hospital, Department of Obstetrics and Gynecology, College of Medicine, Pochon CHA University;Infertility Medical Center, CHA General Hospital, Department of Obstetrics and Gynecology, College of Medicine, Pochon CHA University;Infertility Medical Center, CHA General Hospital, Department of Obstetrics and Gynecology, College of Medicine, Pochon CHA University;
Objective: The present study was conducted to evaluate the viability of germ cells from the adult and fetal ovarian tissues after vitrification followed by xenografting. Method: The human adult ovarian tissues were obtained from 33 years old patient, and the fetal ovarian tissues were obtained from 22 weeks and 25 weeks in gestation. Ovarian tissues were cryopreserved by vitrification with 5.5 M ethylene glycol (EG 5.5) and 1.0 M sucrose as cryoprotectants. Adult and fetal ovarian tissues were pre-equilibrated with EG 5.5 at room temperature for 10 and 5 minutes, respectively and plunged into liquid nitrogen immediately. Frozen-thawed tissues were xenografted into NOD-SCID mice to evaluate the viability and capacity for further growth of the primordial follicles. Grafts were recovered from the recipients 4 weeks after transplantation and histological analysis was accomplished. Result and Conclusion: Grafts recovered 4 weeks after transplantation contained less number of oocytes and primordial follicles compared to that of the fresh tissues. Survived follicles were mainly primordial and intermediary with larger diameter and more granulosa cells. It is confirmed that 1) the ovarian tissues were healthy and the germ cells were survived after vitrification, and 2) the survived fetal primordial follicles after vitrification resumed the growth in the xenografts.
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