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대한생식의학회지 제27권 제2호 2010년
난자채취 2일과 5일에 연속으로 실시한 배아이식의 안전성과 효과
경북대학교병원 산부인과학교실, 대구대학교 축산학과;대구대학교 축산학과;경북대학교병원 산부인과학교실;경북대학교병원 산부인과학교실;
박기상;송해범;이택후;전상식;,
Subsequent Embryo Transfers (SET) on Day 2 and Day 5: It's Safety and Effectiveness
Park, Kee-Sang;Song, Hai-Bum;Lee, Taek-Hoo;Jeon, Sang-Sik;
Department of Obstetrics and Gynecology, Kyungpook National University Hospital, Department of Animal Science, Teagu University;Department of Animal Science, Teagu University;Department of Obstetrics and Gynecology, Kyungpook National University Hospital;Department of Obstetrics and Gynecology, Kyungpook National University Hospital;
Objective: In vitro fertilization (IVF) and a prolonging the time of culture may be helpful in establishing a viable pregnancy through a selection effect. Some embryos do not develop beyond the 4-cell stage and some may not develop to the blastocyst stage. We have evaluated the safety of SET and the outcomes of pregnancy. Methods: Sperms were treated with Ham's F-10 supplemented with 10% human follicular fluid (hFF). oocytes or fertilized oocytes were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10% or 20% hFF respectively. Up to five oocytes were inseminated with approximately 200,000 sperm cells/2 ml in each well. Fertilization was examined in the following morning and fertilized oocytes were co-cultured until embryo transfer. Vero cells for co-culture were prepared in Tissue Culture Medium - 199 (TCM-199) with 10% fetal bovine serum. At the two to four cell and blastocyst on day 2 and day 5, embryo and blstocyst grading were evaluated. Pregnancy rate was determined after transfer of human embryos at the two to four cell stage on day 2 (Group I) or subsequent transfer of embryos on day 2 and at the blastocyst stage on day 5 (Group II). For statistical analysis, Student's t-test and Chi-square (
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