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대한생식의학회지 제27권 제2호 2010년
동결 전 단계적 노출처리방법이 유리화동결 및 초급속동결-융해 후 생쥐 성숙난자의 생존력에 미치는 영향에 관한 연구
부산대학교 의과대학 산부인과학교실;부산대학교병원 불임클리닉;부산대학교병원 불임클리닉;부산대학교 의과대학 산부인과학교실;부산대학교 의과대학 산부인과학교실, 부산대학교병원 불임클리닉;부산대학교 의과대학 산부인과학교실;
김상우;이재익;김미경;이영아;이규섭;윤만수;,
Effects of the Stepwise Exposure Treatments Before Freezing on the Survival Capacity of the Frozen-Thawed Mouse Mature Oocytes by Vitrification or Ultra-Rapid Freezing
Kim, Sang-Woo;Lee, Jae-Ik;Kim, Mi-Kyung;Lee, Young-Ah;Lee, Kyu-Sup;Yoon, Man-Soo;
Department of Obstetrics and Gynecology, College of Medicine, Pusan National University;Infertility Clinic, Pusan National University Hospital;Infertility Clinic, Pusan National University Hospital;Department of Obstetrics and Gynecology, College of Medicine, Pusan National University;Department of Obstetrics and Gynecology, College of Medicine, Pusan National University, Infertility Clinic, Pusan National University Hospital;Department of Obstetrics and Gynecology, College of Medicine, Pusan National University;
Objective: This study was carried out to compare the effects of the stepwise exposure treatments on the morphological normality, fertilization and blastocyst formation rate of the frozen-thawed mouse mature oocytes by vitrification or ultra-rapid freezing and to use as a fundamental data for the cryopreservation of human oocytes. Materials and Methods: The morphological normality and fertilization rates of the vitrified and ultra-rapid frozen mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were observed. After choosing the 3step exposure treatment groups, we observed the morphological normality and fertilization, blastocyst formation rate of the vitrified and ultra-rapid frozen mouse mature oocytes. Results: The morphological normality and fertilization rates of the vitrified mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were 75%, 85%, 88% and 58%, 61 %, 54% respectively. There were no significant differences among treatments(p>0.05). The morphological normality and fertilization rate of the control was 92% and 65%. There were no significant differences in fertilization rate among control and treatments (p>0.05). The morphological normality and fertilization rates of the ultra-rapid frozen mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were 83%, 83%, 84% and 75%, 63%, 56% respectively. There were no significant differences among treatments (p>0.05). The morphological normality and fertilization rate of the control was 95% and 67%. There were no significant differences among control and treatments (p>0.05). The morphological normality and fertilization rate of the vitrified or ultra-rapid frozen mouse mature oocytes after 3step exposure treatment were 69% and 75%, respectively. The blastocyst formation rate was 60% and 57%. The results did not differ significantly between vitrification and ultra-rapid freezing (p>0.05). Conclusion: As known in the above results, there were no significant differences in the fertilization and blastocyst formation rate of the frozen-thawed mouse mature oocytes by vitrification or ultra-rapid freezing among the control and treatments. It is suggested that vitrification and ultra-rapid freezing method were effective for the cryopreservation of mouse mature oocytes.
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