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대한생식의학회지 제27권 제4호 2010년
사람 Y 염색체 특이 DNA Probe의 개발과 이를 이용한 FISH 기술의 임상적 적용
진주산업대학교 동물생명과학과, 세화산부인과 불임/유전연구소;세화산부인과 불임/유전연구소;세화산부인과 불임/유전연구소;세화산부인과 불임/유전연구소;진주산업대학교 동물생명과학과;진주산업대학교 동물생명과학과;
류란숙;이상찬;이채식;김종흥;류은경;손시환;,
Identification of a Human Y Chromosome Specific DNA Probe and Their Clinical Application by Fluorescence in situ Hybridization Techniques
Ryu, Ran-Suk;Lee, Sang-Chan;Lee, Chae-Sik;Kim, Jong-Heung;Ryu, Eun-Koung;Sohn, Sea-Hwan;
Department of Animal Science & Biotechnology, Chinju National University, SaeWha Obstetrics and Gynecology;SaeWha Obstetrics and Gynecology;SaeWha Obstetrics and Gynecology;SaeWha Obstetrics and Gynecology;Department of Animal Science & Biotechnology, Chinju National University;Department of Animal Science & Biotechnology, Chinju National University;
Objective: The present study was undertaken to synthesize a human Y chromosome specific probe and to confirm the usefulness of the probe for fluorescence in situ hybridization (FISH) in various types of human cells. Methods: An approximately 400 bp DNA fragment of the DYZ1 sequences was synthesized by PCR using digoxigenin labeled dUTP (dig-PCR). The fidelity of probe was tested by FISH for cultured and uncultured human lymphocytes, amniocytes, chorionic villus cells, embryos, sperms, and germ cells of seminiferous tubule. Results: The human Y chromosome specific probe hybridized specifically to Y chromosome of the cells that had been tested. This probe assigned to the Yq12 region where the DYZ1 repetitive sequence is concentrated. Conclusion: We have identified a human Y chromosome specific probe that hybridized specifically to the Y chromosome by FISH for various types of uncultured as well as cultured cells. Therefore FISH technique using human Y chromosome specific probe should be useful for clinical application as a diagnostic tool for the detection of human Y chromosome.
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