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대한생식의학회지 제26권 제2호 2010년
Phosphate가 제거된 단순배양액중 아미노산의 첨가가 체외수정시술후 임신율에 미치는 영향
홍산부인과1, 혜성산부인과병원2, 신아산부인과 불임클리닉3
이지삼1, 홍정의1, 유승환2, 정구성2, 홍기언2, 전은숙3, 허영문3, 이종인3 ,
Effects of Amino Acids in Simple Phosphate-Free Media on Pregnancy Rate in Human In Vitro Fertilization and Embryo Transfer(IVF-ET)
Lee js, Hong je, Yoo sh, Jung gs, Hong ke, Jeon es, Hur ym, Lee ji
The role of amino acids in culture media for IVF-ET was examined in a total of 68 patients with 76 cycles. Patients received clomiphene citrate(CC) followed by hMG or GnRH-a combined with gonadotropins(FSH/hMG) for controlled ovarian hyperstimulation. Severe male(<4 X 106 motile sperm) or age factor(>39 y) patients were excluded in this study. Pregnancy was classified as clinical if a gestational sac or fetal cardiac activity was seen on ultrasound. The mean number of exogenous gonadotrpins was not different between bHTF(without amino acids) and mHTF(with amino acids) groups. No significant differences were found in age, duration of infertility, follicle size, the level of E2 on the day of hCG injection, the mean number of oocytes retrieved, total motile sperm count, fertilization rate and the mean number of embryos transferred. However, total ampules of gonadotropins were higher(P<0.01) in mHTF group than in bHTF group. Significantly (P<0.05) more clinical pregnancies were resulted from mHTF group(13/30) compared with bHTF group(9/46). The multiple pregnancy rates were 11.1% in bHTF group and 7.7% in mHTF group. There were one ectopic pregnancy in mHTF group and one heterotopic pregnancy in bHTF group. Abortion rates were 22.2% in bHTF group and 7.7% in mHTF, respectively. The ongoing pregnancy or livebirth rate was significantly(P<0.05) higher in mHTF group (12/30) than in bHTF group(7/46). These results suggest that the addition of amino acids in culture media is essential for culture of zygotes in vitro and adjustment of energy substrates in phosphate-free culture media appear to be beneficial for human IVF-ET procedure.
키워드 : Embryo culture, Amino acids, Pregnancy, IVF-ET
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